Analysis of biocontrol Actinomycetes BM-25 identification,biological characteristics and control efficacy
Antagonistic strains of tobacco root black rot caused by Thielaviopsis basicola were isolated and screened from tobacco rhizosphere soil,and their antibacterial activity,morphological,biochemical,and molecular identification were conducted.The biocontrol effect of antagonistic strains was investigated by pot experiment.The results showed that the antibacterial rate of BM-25 isolated from tobacco rhizosphere soil was 52.78%,which showed broad-spectrum antibacterial activity.Based on the morphological,biochemical and 16S rRNA sequence characteristics,BM-25 was initially identified as a Nocardiopsis strain,and named Nocardiopsis sp.BM-25.The bioactivity of strain BM-25 showed that it had the ability to solubilize phosphorus and potassium,and produced IAA,cellulolytic enzyme and amylase.The pot experiment results indicated that compared to the CK treatment,the strain BM-25 treatment increased the tobacco plant height by 49.72%,stem diameter by 44.53%,and the largest leaf area by 73.58%.The above results indicated that strain BM-25 had good biocontrol and growth promotion effects on tobacco,and could be used as a candidate genetic resource for the development of tobacco-specific bactericides.
NETL
NSTL
万方数据
