Effects of Hartz-Bacillus subtilis on the growth and accumulation of active substances in the bulbs of Lilium davidii var.unicolor
To explore the effects of different application rates of Hartz-Bacillus subtilis on the growth and development of Lilium davidii var.unicolor,as well as the accumulation of active substances in the bulbs,and to screen for the optimum application rate of Hartz-Bacillus subtilis for the high-quality production of L.davidii var.unicolor.Regarded L.davidii var.unicolor was uesd as material,on the basis of a defined organic fertilizer application rate of chicken manure,1 blank control(CK)and 5 treatments of Hartz-Bacillus subtilis(15,30,45,60 and 75 kg/hm2)were constructed.The agronomic traits such as plant height and stem thickness of its and the content of polysaccharide,saponin,and other active substances in the bulb were analyzed by measuring the main agronomic traits of L.davidii var.unicolor and the content of active substances in the bulb under different treatments.The results showed that Hartz-Bacillus subtilis application accelerated the growth and increased the content of active substances of this plant.When applied at a rate of 75 kg/hm2,the agronomic traits showed good performance,with stem thickness,leaf number,chlorophyll content,and bulb circumference increasing by 22.21%,22.11%,18.16%,and 8.46%,respectively,compared to CK.The plant height was 1.37 times that of CK.In terms of the content of active substances in the bulbs,the polysaccharide and saponin content were highest when applied at a rate of 75 kg/hm2,increasing by 68.85%and 59.80%respectively compared to CK.The total phenolic content was highest when applied at a rate of 60 kg/hm2.Overall,in the cultivation of L.davidii var.unicolor,the optimal application rate of Hartz-Bacillus subtilis was 75 kg/hm2.The research results provide a reference for the application of microbial agents in high-quality cultivation of L.davidii var.unicolor.
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