Comparison of pollen vitality and storage conditions among three varieties of raspberry
In this study,3 species of raspberry were used as test materials,such as Rubus eustephanus Focke ex Diels,Rubus fruticosus Pollich,and Rubus chingii Hu.Pollen viability at different development stages(flowering stage I,II,III,IV),different time periods(8:30,10:30,12:30,14:30,16:30),different drying conditions(natural drying method and silica gel drying method),and different storage conditions(4 and 20℃)were measured.The stigma receptivity of raspberry pollen at different developmental stages was determined.To screen out the appropriate pollen collection,treatment time,and storage conditions.The results showed that the best pollen collection period of raspberry was flowering stage Ⅲ,the best pollination period of hybridization was flowering stage Ⅲ,and the best emasculation period was flowering stage Ⅰ and Ⅱ.The pollen activity of raspberry decreased at noon,and the best time period was 12:30-14:30.The pollen viability of silica gel was better preserved after drying for 24 h.When the water content of Rubus eustephanus Focke ex Diels,Rubus fruticosus Pollich,and Rubus chingii Hu was 20.59%,14.67%and 15.36%,respectively,the storage resistance was better,and could be stored for 90 days at-20℃.The results provide a reference for the work of hybridization and breeding of raspberry.
raspberrypollen vitalitystigma receptivitylow temperature storage
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