Research on in vitro culture of miniature Chinese rose'Lingzhi Yaojing'
In order to promote the large-scale seedling production and subsequent germplasm improvement by cell engineering methods,the goblins of miniature Chinese rose'Lingzhi Yaojing'were used as experimental materials to study in vitro culture,including different explants disinfection and sterilization,callus induction,callus proliferation,axillary bud germination induction,axillary bud subculture proliferation,and rooting induction.The results showed that the suitable sterilization method for leaf explants was 75%ethanol disinfection for 30 s+0.1%mercury chloride sterilization for 5 min,and the suitable callus induction medium was MS+6-BA 0.5 mg/L+2,4-D 2.0 mg/L+sucrose 30 g/L+agar 6.5 g/L.Callus proliferation medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 6.5 g/L.The appropriate sterilization method for stem explants was 75%ethanol disinfection for 30 s+0.1%mercury chloride sterilization for 8 min.The medium suitable for axile germination was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.The subculture proliferation medium for axillary bud was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.The suitable medium for rooting was 1/2MS+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.This study provides a reference for the improvement and genetic transformation of microrose strains.
miniature Chinese rosein vitro culturecallus inductionsubculture proliferation
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