微型月季'玲之妖精'的离体培养研究
Research on in vitro culture of miniature Chinese rose'Lingzhi Yaojing'
方荷芳 1周鑫平 1康旭东 1彭晓龙 1李同建 1贾明良1
作者信息
摘要
为促进微型月季'玲之妖精'的种苗规模化生产以及后续利用细胞工程方法进行种质改良,以微型月季'玲之妖精'为试验材料,利用组织培养方法进行离体培养研究,主要包括不同外植体消毒灭菌处理、愈伤组织诱导、愈伤组织增殖、腋芽萌发诱导、腋芽继代增殖以及生根诱导等.结果表明,叶片外植体合适的灭菌方式为75%乙醇消毒30 s+0.1%升汞灭菌5 min,适合的愈伤组织诱导培养基为MS+6-苄基腺嘌呤(6-BA)0.5 mg/L+2,4-二氯苯氧乙酸(2,4-D)2.0 mg/L+蔗糖30 g/L+琼脂6.5 g/L,愈伤组织增殖培养基为MS+6-BA 2.0 mg/L+萘乙酸(NAA)0.2 mg/L+蔗糖30 g/L+琼脂6.5 g/L;茎段外植体合适的灭菌方式为75%乙醇消毒30 s+0.1%升汞灭菌8 min,适合腋芽萌发的培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂6.5 g/L,腋芽继代增殖的培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂6.5 g/L;生根适宜的培养基为1/2MS+NAA 0.1 mg/L+蔗糖 30 g/L+琼脂 6.5 g/L.本研究为微型月季品系改良和遗传转化提供参考.
Abstract
In order to promote the large-scale seedling production and subsequent germplasm improvement by cell engineering methods,the goblins of miniature Chinese rose'Lingzhi Yaojing'were used as experimental materials to study in vitro culture,including different explants disinfection and sterilization,callus induction,callus proliferation,axillary bud germination induction,axillary bud subculture proliferation,and rooting induction.The results showed that the suitable sterilization method for leaf explants was 75%ethanol disinfection for 30 s+0.1%mercury chloride sterilization for 5 min,and the suitable callus induction medium was MS+6-BA 0.5 mg/L+2,4-D 2.0 mg/L+sucrose 30 g/L+agar 6.5 g/L.Callus proliferation medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 6.5 g/L.The appropriate sterilization method for stem explants was 75%ethanol disinfection for 30 s+0.1%mercury chloride sterilization for 8 min.The medium suitable for axile germination was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.The subculture proliferation medium for axillary bud was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.The suitable medium for rooting was 1/2MS+NAA 0.1 mg/L+sucrose 30 g/L+agar 6.5 g/L.This study provides a reference for the improvement and genetic transformation of microrose strains.
关键词
微型月季/离体培养/愈伤组织诱导/继代增殖Key words
miniature Chinese rose/in vitro culture/callus induction/subculture proliferation引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据