Advances in the application of soy lecithin and low-density lipoprotein in the cryopreservation of goat semen
Semen cryopreservation technology is an important means for the protection of genetic resources and breeding management in modern animal husbandry.In this paper,the research progress of soy lecithin(SL)and low-density lipoprotein(LDL)in cryopreservation of goat semen was discussed from the aspects of cryopreservation technology,cryopreservation damage in semen cryopreservation,and application of cryoprotectants,the mechanism and application of SL and LDL in cryopreservation of semen,and their combined application.The cryopreservation technology of goat semen is to make the physiological activity of sperm in goat semen static by constructing an extremely low temperature environment.Semen is affected by low temperature environment during storage,resulting in cell damage and accumulation of reactive oxygen species leading to sperm quality decline.The use of cryopprotectants such as glycerin and yolk can reduce the formation of ice crystals during cryopreservation of semen and protect sperm cell membrane from the punctured ice crystals.SL as a natural phospholipid,can reduce the formation of ice crystals and the damage of reactive oxygen species to sperm during cryopreservation of goat semen,thus reducing the damage of sperm by cryopreservation.LDL as a lipoprotein in egg yolk,can competitively bind with harmful cationic peptides in semen to protect sperm from harmful protein damage.The two have a synergistic effect,LDL enhances the cryoprotective effect of SL,while SL provides additional antioxidant protection,which together improves the survival rate of sperm during freezing and thawing,improving the overall effect of the cryoprotectants.This paper provides ideas and references for cryopreservation of goat semen.
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维普
