Effects of graphene oxide on seed germination and seedling growth of Acacia cincinnata
In order to analyze the effects of different concentrations of graphene oxide(GO)on seed germination and seedling growth of Acacia cincinnata,and to screen suitable treatment methods to improve seeding quality of Acacia cincinnata varieties,the new seeds collected in the same year and the old seeds stored for 5 years were studied with GO concentrations of 0(CK),25,50,100,200,400 and 800 mg/L,respectively,and the germination rate,seedling height growth index and the accumulation of contents such as soluble sugar were measured under different treatments.The results showed that the seed germination index,seedling growth and inclusions accumulation showed different trends with the change of GO treatment concentration.The germination rate and germination potential of new and old seeds were the best under 100 mg/L GO treatment.The plant height,fresh weight,soluble sugar and total protein contents of seedlings under 200 mg/L GO treatment had the highest increase compared with CK treatment.In conclusion,100-200 mg/L GO treatment can effectively promote the germination,seedling growth and content accumulation of new and old seeds of this tree species.This study provides a reference for improving the breeding methods of Acacia cincinnata.