Objective:To preliminarily investigate the effects of maternal thyrotoxicosis and antithyroid drug intervention on offspring cognition by establishing a female pregnant thyrotoxic rat model. Methods:Female SD rats were selected and randomly divided into control and hyperthyroid groups, in which the hyperthyroid group was divided into hyperthyroid non-intervention, early intervention and mid-late intervention groups after determining the success of modelling. The hyperthyroidism model was established by gavage with levothyroxine sodium suspension 0.5 mg/kg, and the learning and memory abilities of the young rats were determined by the Morris water maze (MYM) experiment. Hippocampal morphology, expression of mature neuronal nucleoprotein (NeuN) and synapse-associated protein (SYN) were observed by haematoxylin and eosin (HE) staining, Nysted staining and immunohistochemistry in young rats. Results:HE staining: significant neuronal loss was observed in the hyperthyroidism non-intervention group, the early intervention group and the mid-late intervention group. Nysted staining: compared with the control group, there was significant statistical significance in the hyperthyroidism non-intervention group and the mid-late intervention group (P<0.001), and compared with the hyperthyroidism non-intervention group, there was significant statistical significance in the early intervention group and the mid-late intervention group (P<0.05). Water maze experiment: There was no significant statistical significance in the avoidance latency to reach the platform, the dwell time in the quadrant where the platform was located, and the number of times the original platform was crossed in each group (P>0. NeuN expression level: The mean optical density of NeuN-positive cells in the CA1 region of hippocampal tissue was significantly lower in the hyperthyroidism non-intervention group and the early intervention group compared with the control group (P<0.05).SYN expression level: The mean optical density of SYN in the CA1 region of hippocampal tissue was significantly lower in the hyperthyroidism groups of young rats compared with the control group (P<0.05). Compared with the hyperthyroid non-intervention group, the mean optical density of SYN in the CA1 region of hippocampal tissue was significantly lower in the early intervention group and the mid-late intervention group (P<0.05). Conclusion:Gestational thyrotoxicosis may adversely affect offspring cognitive function and neuronal development, and early intervention and treatment may improve the effects on offspring cognitive function and neuronal development.
thyrotoxicosisoffspringhippocampusmorris water maze experiment
维普
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