Objective:The aim of this study was to investigate the current state,weaknesses and influencing factors of patient safety culture among medical staff in a tertiary comprehensive hospital.Methods:A cross-sectional survey was conducted among 1,336 healthcare personnel in the hospital using the hospital survey of patient safety culture(HSOPSC)through a convenience sampling method.Results:The overall score for patient safety culture in the hospital was 3.78±0.51,with an overall positive response rate of 66.65%.There were five strengths identified in the patient safety culture,including teamwork within departments(82.49%),managers'willingness and actions to promote safety(79.06%),organizational learning and continuous improvement(86.73%),management support for patient safety(78.84%),and feedback and communication about errors(79.49%);areas requiring improvement were identified as staffing(39.78%)and non-punitive response to errors(41.79%).Conclusion:Factors that influenced the patient safety culture in the hospital included administrative position,average daily overtime hours,and awareness of a non-punitive reporting system(P<0.05).Rational allocation of human resources and fostering a non-punitive culture are crucial measures for enhancing patient safety culture.