Objective:To investigate the therapeutic effects of Polygonatum sibiricum polysaccharides(PSP)on oral ulcer model rats and its mechanism.Methods:A total of 60 rats were randomly divided into the normal group,model group and PSP low-dose(50 mg/kg),medium-dose(100 mg/kg),and high-dose(200 mg/kg)groups.Except for the normal group,all the rats were mechanically traumatized and burned with sodium hydroxide(NaOH)crystals to establish oral ulcer models.After modeling,the drugs were administered by continuous gavage for 10 days.Normal saline was administered to the normal and model groups,while the low,medium,and high dose PSP groups were respectively administered with corresponding concentrations of PSP solution.The maximum transverse and longitudinal diameters of oral ulcers were measured before administration and on the 3rd,5th,7th and 10th days of administration in each group,and the area of ulcers was calculated.The levels of tumor necrosis factor(TNF)-α and interleukin(IL)-6 were measured using enzyme-linked immunosorbent assay(ELISA),and the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)were measured using the water-soluble tetrazolium blue method(WST)and the thiobarbituric acid method(TBA),respectively,in the serum of rats.Results:The reduction of ulcer area was more significant in the PSP drug group,especially in the PSP medium-and high-dose groups,compared to the model group(P＜0.05).The PSP low-,medium-,and high-dose groups significantly reduced the elevated serum levels of TNF-α,IL-6,and MDA in the modeled rats(P＜0.05),as well as increased the serum SOD level(P＜0.05).Conclusion:PSP exhibited a significant therapeutic effect on oral ulcer model rats induced by mechanochemical chemical association method,which could significantly promote the healing of oral ulcer lesions.The treatment mechanism may be related to its anti-inflammatory and antioxidant effects.
NETL
NSTL
万方数据
