Objective:To explore the effects of breviscapine in myocardial protection and its molecular mechanism in rats with acute myocardial infarction(AMI).Methods:Thirty-two SD rats were randomly and equally divided into sham-operated group,AMI group,AMI+breviscapine group,AMI+breviscapine+LY294002 group,8 rats in each group.Echocardiography,TUNEL staining,ELISA,Western blot and qRT-PCR were used to investigate the cardiac function,myocardial apoptosis rate,myocardial injury degree,the expression of apoptosis-related molecules and PI3K/Akt signaling pathway in rats of each group.Results:Compared with the sham-operated group,rats in the AMI group showed reduced cardiac contractile function,significantly increased apoptosis rate,severe myocardial injury,while compared with the AMI group,the AMI+breviscapine group showed the above pathological changes were significantly reduced(P＜0.05);when LY294002,a specific inhibitor of PI3K,was added,the therapeutic effect of breviscapine on AMI was diminished.Conclusion:Breviscapine may inhibit cardiomyocyte apoptosis through activation of PI3K/Akt signaling pathway,thereby reducing myocardial injury caused by AMI and improving cardiac function.
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