灯盏花素对急性心肌梗死大鼠的心肌保护作用及机制研究
Myocardial protective effect and mechanism of Breviscapine in rats with acute myocardial infarction
李浩亮 1许邦龙 1胡广全 1高峰1
作者信息
- 1. 安徽医科大学第二附属医院心血管内科 安徽合肥 230601
- 折叠
摘要
目的:探讨灯盏花素对急性心肌梗死(AMI)大鼠的心肌保护作用及其分子机制.方法:将32只SD大鼠随机均分为假手术组、AMI组、AMI+灯盏花素组,AMI+灯盏花素+LY294002组.通过超声心动图、TUNEL染色、ELISA、Western blot和qRT-PCR等多种检测方法,探究各组大鼠的心功能、心肌细胞凋亡率、心肌损伤程度、凋亡相关分子以及PI3K/Akt信号通路的表达情况.结果:与假手术组比较,AMI组大鼠的心脏收缩功能降低,心肌细胞凋亡率显著增加,心肌损伤严重;与AMI组比较,AMI+灯盏花素组的上述病理改变明显减轻;当加入PI3K的特异性抑制剂LY294002后,灯盏花素对AMI大鼠的治疗作用被削弱.结论:灯盏花素可能是通过激活PI3K/Akt信号通路抑制心肌细胞凋亡,从而减轻AMI导致的大鼠心肌损伤,改善心功能.
Abstract
Objective:To explore the effects of breviscapine in myocardial protection and its molecular mechanism in rats with acute myocardial infarction(AMI).Methods:Thirty-two SD rats were randomly and equally divided into sham-operated group,AMI group,AMI+breviscapine group,AMI+breviscapine+LY294002 group,8 rats in each group.Echocardiography,TUNEL staining,ELISA,Western blot and qRT-PCR were used to investigate the cardiac function,myocardial apoptosis rate,myocardial injury degree,the expression of apoptosis-related molecules and PI3K/Akt signaling pathway in rats of each group.Results:Compared with the sham-operated group,rats in the AMI group showed reduced cardiac contractile function,significantly increased apoptosis rate,severe myocardial injury,while compared with the AMI group,the AMI+breviscapine group showed the above pathological changes were significantly reduced(P＜0.05);when LY294002,a specific inhibitor of PI3K,was added,the therapeutic effect of breviscapine on AMI was diminished.Conclusion:Breviscapine may inhibit cardiomyocyte apoptosis through activation of PI3K/Akt signaling pathway,thereby reducing myocardial injury caused by AMI and improving cardiac function.
关键词
灯盏花素/急性心肌梗死/PI3K/Akt信号通路/细胞凋亡Key words
breviscapine/acute myocardial infarction/PI3K/Akt signaling pathway/apoptosis引用本文复制引用
基金项目
安徽省高等学校科学研究项目(2022AH050771)
出版年
2024
NETL
NSTL
万方数据