大麻素2型受体在加速正畸牙移动中的作用
The role of CB2 in accelerating orthodontic tooth movement
范登莹 1翟浩嫣 1刘慧娟 2赵圆 1李东娜 1乔星 1康文静 1朱德超 1刘春艳1
作者信息
- 1. 河北医科大学口腔医(学)院正畸科,石家庄 050017
- 2. 河北医科大学口腔医(学)院重点实验室,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,石家庄 050017
- 折叠
摘要
目的 探讨大麻素2型受体(CB2)在正畸过程中对小鼠正畸牙移动(OTM)速率和压力侧牙周组织改建的作用.方法 筛选CB2-/-和同窝WT型各30只雄性小鼠,6周龄时建立牙齿移动模型,建模时间分别为0、3、7、14、21 d(n=6)后取材,经体视显微镜测量牙移动距离;HE染色观察根压力区牙周组织变化;TRAP染色统计根压力区破骨细胞数量;免疫组织化学染色观察根压力区基质金属蛋白酶-9(MMP-9)阳性单核细胞和多核细胞的数量变化.结果 正畸牙移动距离测量结果显示,在21 d内,随着时间延长,牙齿移动距离逐渐增加,且3、7、14、21 d时CB2-/-组牙齿移动距离均较WT小鼠增加;HE染色显示OTM第14天时CB2/-小鼠压力区牙周膜间隙宽度大于WT小鼠(P＜0.000 1);TRAP染色显示14 d时,在第一磨牙远中根压力区硬骨板处,CB2-/-小鼠的破骨细胞数量大于WT小鼠(P＜0.001);MMP-9免疫组织化学染色显示OTM 14 d时,在压力区牙周膜区域,CB2-/-小鼠的MMP-9(+)单核细胞和MMP-9(+)多核细胞数量均大于WT小鼠(P＜0.05).结论 CB2可加速正畸力作用下牙齿移动速率.CB2缺失加速正畸牙移动是通过加速牙移动过程中压力侧骨吸收实现的.
Abstract
Objective To explore the effect of cannabinoid receptor 2(CB2)on orthodontic tooth movement(OTM)rate and periodontal tissue reconstruction of pressure area in mice.Methods Thirty CB2-/-male mice and thirty littermate control WT male mice were individually accepted the orthodontic appliance at their age of 6 weeks.The mice were respectively scarified at 3 days,7 days,14 days and 21 days after the operation.Then the tooth movement distance was examined through the stereomicroscope.Hematoxylin-eosin staining was performed to explore the biological responses of periodontium at the distal mesial root pressure area.Anti-tartrate acid phospha-tase staining was performed to calculate the number and distribution of osteoclasts at the distal mesial root pressure area,and MMP-9 was evaluated by immunohistochemistry to examine the number of MMP-9(+)monocytes and multinucleated cells in the same district as the TRAP staining.Results Compared with those WT mice at 3,7,14 and 21 days,OTM distance showed a gradual increased tendency according with experimental time over 21 days.The widths of periodontal ligament on the pressure side were markedly greater in CB2-/-mice than WT mice at 7,14 and 21 days(P＜0.000 1).The numbers of TRAP positive osteoclasts were significantly greater in CB2-/-mice than those in WT mice at 14 days of OTM(P＜0.001).MMP-9 immunohistochemical staining showed that the number of MMP-9(+)monocytes and multinucleated cells was more in CB2-/-mice than that in WT mice at 14 days of OTM(P＜0.05).Conclusion The absence of CB2 accelerates orthodontic tooth movement under or-thodontic force.The absence of CB2 reinforces bone resorption in orthodontic tooth movement compressive area dur-ing orthodontic tooth movement.
关键词
大麻素2型受体/正畸牙移动/压力区/骨改建/破骨细胞Key words
cannabinoid receptor 2/orthodontic tooth movement/compressive area/bone remodelling/osteoclast引用本文复制引用
基金项目
政府资助临床医学优秀人才项目(2021)(361029)
引进留学人员资助项目(2022)(C20220353)
出版年
2024
国家科技期刊平台
NSTL
万方数据