GNB4和Riplet基因甲基化检测在原发性肝癌诊断中的价值研究
The value of GNB4 and Riplet gene methylation detection in the diagnosis of primary liver cancer
杨玉萍 1徐恩君 1汪轩轩 1唐宜桂 1郑美娟 1王悦 1储梦真 1徐家丹 1王中新1
作者信息
- 1. 安徽医科大学第一附属医院检验科 合肥 230022
- 折叠
摘要
目的 探讨GNB4和Riplet基因甲基化单独和联合检测在原发性肝癌诊断中的诊断效能和临床价值.方法 选取313例患者,原发性肝癌患者78例,其他消化系统肿瘤患者41例,非消化系统肿瘤患者17例,肝癌术后20例,肝良性疾病患者157例.采用甲基化荧光定量PCR(qMSP)法检测所有患者血浆中GNB4和Riplet基因甲基化水平,直接化学发光法检测血清甲胎蛋白(AFP)水平.结果 AFP检测诊断灵敏度和特异度为51.3％、94.3％;GNB4基因甲基化检测诊断灵敏度和特异度为83.3％、99.4％;Riplet基因甲基化检测诊断灵敏度和特异度为73.1％、99.4％.GNB4和Riplet基因甲基化联合检测诊断的灵敏度和特异度为92.3％、98.7％;AFP、GNB4和Riplet基因甲基化3项指标联合检测诊断的灵敏度和特异度为92.3％、98.7％,纳入年龄和性别后的联合诊断的灵敏度和特异度为93.6％、97.5％.结论 对于原发性肝癌的诊断,AFP灵敏度和特异度存在局限性,而GNB4和Riplet基因甲基化水平较高,两者联合检测的灵敏度和特异度均高于AFP的诊断效能.AFP、GNB4和Riplet基因甲基化联合检测诊断的灵敏度和特异度显著高于AFP、GNB4和Riplet基因甲基化单独检测.
Abstract
Objective To investigate the diagnostic efficacy and clinical value of GNB4 and Riplet gene methylation alone and in combination in the diagnosis of primary liver cancer.Methods A total of 313 patients were selected,including 78 patients with primary liver cancer,41 patients with other digestive system tumors,17 patients with non-digestive system tumors,20 patients with postoperative liver cancer,and 157 patients with benign liver disea-ses.The levels of GNB4 and Riplet gene methylation in plasma were detected using quantitative methylation-specific PCR(qMSP).Serum alpha-fetoprotein(AFP)levels were measured by direct chemiluminescence.Results The sensitivity and specificity of AFP in diagnosis were 51.3％and 94.3％,respectively;the sensitivity and specificity of GNB4 gene methylation in diagnosis were 83.3％and 99.4％,respectively;the sensitivity and specificity of Riplet gene methylation in diagnosis were 73.1％and 99.4％,respectively.The sensitivity and specificity of GNB4 and Riplet gene methylation combined diagnosis were 92.3％and 98.7％,respectively;the sensitivity and specificity of AFP,GNB4 and Riplet gene methylation combined diagnosis were 92.3％and 98.7％,respectively;the sensitivity and specificity of combined diagnosis including age and gender were 93.6％and 97.5％,respective-ly.Conclusion The sensitivity and specificity of AFP in the diagnosis of primary liver cancer are limited,while the methylation levels of GNB4 and Riplet genes are higher,and the sensitivity and specificity of their combined de-tection are higher than those of AFP.The sensitivity and specificity of AFP,GNB4 and Riplet gene methylation combined diagnosis are significantly higher than those of AFP,GNB4 and Riplet gene methylation alone.
关键词
原发性肝癌/甲胎蛋白/基因甲基化/GNB4基因/Riplet基因Key words
primary liver cancer/alpha-fetoprotein/gene methylation/GNB4 gene/Riplet gene引用本文复制引用
基金项目
安徽省高等学校自然科学研究项目(2023AH020048)
出版年
2024
国家科技期刊平台
NSTL
万方数据