首页|乙醇脱氢酶1A和血管内皮生长因子-A在肝细胞癌中的表达

乙醇脱氢酶1A和血管内皮生长因子-A在肝细胞癌中的表达

Expression of alcohol dehydrogenase 1 A and vascular endothelial growth factor-A in hepatocellular carcinoma

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目的 探讨乙醇脱氢酶 1A(ADH1A)和血管内皮生长因子-A(VEGFA)在肝细胞癌(HCC)中的表达和临床意义.方法 通过基因表达谱交互(GEPIA)分析 ADH1A和VEGFA在HCC以及癌旁正常组织中的表达情况及相关性;肿瘤基因组图谱(TCGA)和基因富集分析(GSEA)探讨ADH1A在HCC中的相关通路;收集 84 例HCC患者的样本及临床病理资料,并选取54 例癌旁正常组织样本做对照,分析ADH1A和VEGFA与HCC中临床病理参数的相关性;采用免疫组化法检测并分析病例组和对照组ADH1A和VEG-FA的蛋白表达情况,结合临床病理参数并通过Kaplan-Meier法分析ADH1A和VEGFA的表达与HCC患者的临床进展和预后的关系.结果 生物信息学分析发现ADH1A在HCC中低表达而 VEGFA在 HCC中高表达,并且两者呈负相关关系(P<0.001);免疫组化检测结果显示ADH1A在 HCC组织中的表达率低于癌旁正常组织(P<0.01),而 VEGFA在HCC组织中表达率高于癌旁正常组织(P<0.01);HCC组织中ADH1A高表达组的脉管癌栓及HCC患者的复发比例更低(P<0.05);HCC组织中VEGFA高表达组的肿瘤直径>5 cm、高TNM分期、有微卫星以及G2-G3 分化程度比例更高(P<0.05);Kaplan-Meier 生存分析显示,ADH1A高表达同时VEGFA低表达患者的五年生存率较高.结论 HCC患者肿瘤组织中ADH1A的低表达以及VEGFA的高表达与肿瘤的进展有关,可作为HCC患者预后评估指标之一.
Objective To investigate the expression,synergistic relationship and clinical significance of alcohol de-hydrogenase(ADH1A)and vascular endothelial growth factor-A(VEGFA)in hepatocellular carcinoma(HCC).Methods The expression and correlation of ADH1A and VEGFA in HCC and adjacent normal tissues were ana-lyzed by GEPIA.TCGA and GSEA were used to analyze the pathway of ADH1A in HCC.The clinical and patho-logical data of 84 patients with HCC were collected,and 54 patients with paracancer normal tissue samples were se-lected as controls to analyze the correlation between ADH1A and VEGFA and clinicopathological parameters of HCC.Immunohistochemistry was used to detect the protein expression of ADH1A and VEGFA in cases and con-trols,and the correlation between the expression of ADH1A and VEGFA and the clinical progression and prognosis of patients with HCC was analyzed based on clinical pathological parameters and Kaplan-Meier.Results Bioinfor-matics analysis found that ADH1A was low-expressed in HCC and VEGFA was highly expressed in HCC,and there was a negative correlation between the two(P<0.001);immunohistochemical detection results showed that the expression of ADH1A in HCC tissue was lower than that in normal tissue adjacent to cancer(P<0.01)while the expression rate of VEGFA in HCC tissue was significantly higher than that of normal tissue adjacent to cancer(P<0.01);The recurrence rate of vascular thrombus and HCC patients in HCC group with high expression of ADH1A was lower(P<0.05).The proportion of tumor diameter>5 cm,high TNM stage,microsatellite and G2-G3 dif-ferentiation in HCC tissues in VEGFA high expression group was higher(P<0.05).Kaplan-Meier survival analy-sis showed that patients with high ADH1A expression and low VEGFA expression had a higher five-year survival rate.Conclusion Low expression of ADH1A and high expression of VEGFA in tumor tissues of patients with HCC indicate tumor progression and can be used as one of the prognostic evaluation indicators for patients with HCC.

hepatocellular carcinomaalcohol dehydrogenase 1Avascular endothelial growth factor-Atumor re-currencebioinformaticsprognosis

薛乐乐、井玉莹、杨凯歌、祁力文、吴桐、任祎琳、臧翌辰、王良海、张海俊、梁伟华、胡建明

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石河子大学医学院病理学系/石河子大学第一附属医院病理科,石河子 832000

肝细胞癌 乙醇脱氢酶 血管内皮生长因子-A 肿瘤复发 生物信息学 预后

国家自然科学基金兵团指导性科技计划石河子大学医学院第一附属医院临床基础联合基金石河子大学医学院第一附属医院青年基金国家级大学生创新创业训练计划

819604352023ZD027LJ202202QN202003202310759018

2024

10.19405/j.cnki.issn1000-1492.2024.03.020

安徽医科大学学报
安徽医科大学

安徽医科大学学报

CSTPCD北大核心
影响因子：1.095
ISSN：1000-1492
年,卷(期)：2024.59(3)
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