miR-19a靶向胰岛素样生长因子2受体抑制血管瘤干细胞的增殖和迁移
MiR-19a affects hemangioma stem cells proliferation and migration by targeting insulin-like growth factor 2 receptor
汪帆 1吴瑶 2方林森 1曹东升2
作者信息
- 1. 安徽医科大学第一附属医院北区(安徽省公共临床中心)创面修复与整形美容外科,合肥 230001
- 2. 安徽医科大学第二附属医院创面修复与整形美容外科,合肥 230001
- 折叠
摘要
目的 探讨婴儿血管瘤(IHs)中miR-19a是否与胰岛素样生长因子2受体(IGF-2R)相互作用,影响血管瘤干细胞(HemSCs)的增殖、迁移和脂肪生成.方法 从IHs标本中分离、筛选和培养HemSCs,免疫组织化学鉴定IGF-2R在HemSCs中表达.用miR-19a模拟物和抑制剂对HemSCs进行转染,通过CCK-8、划痕实验、Transwell、qRT-PCR和免疫印迹相关实验验证miR-19a对于HemSCs增殖与迁移的影响.结果 与对照组相比,用miR-19a抑制剂处理的Hem-SCs增殖与迁移速度显著增加,miR-19a过表达显著抑制IGF-2诱导的细胞迁移和增殖(P＜0.05).结论 miR-19a可能通过靶向IGF-2R抑制HemSCs的增殖、迁移和脂肪生成.
Abstract
Objective To investigate whether miR-19a interacts with insulin-like growth factor 2 receptors(IGF-2R)in infantile hemangiomas(IHs)and affects the proliferation,migration,and adipogenesis of hemangioma stem cells(HemSCs).Methods HemSCs were isolated,screened and cultured from IH specimens.IGF-2R expression in HemSCs was identified using immunohistochemistry.HemSCs transfected with miR-19a mimics and inhibitors were subjected to CCK-8,wound healing,Transwell,qRT-PCR,and Western blot analyses.Results Compared with the control,the proliferation and migration rate of HemSCs treated with miR-19a inhibitors were significantly increased,and overexpression of miR-19a significantly inhibited IGF-2 induced cell migration and proliferation(P＜0.05).Conclusion MiR-19a may inhibit HemSCs proliferation,migration,and adipogenesis by targeting IGF-2R.
关键词
血管瘤干细胞/微小RNA/IGF-2R/增殖/迁移Key words
hemangioma stem cells/microRNA/IGF-2R/proliferation/migration引用本文复制引用
基金项目
安徽省自然科学基金项目(1808085mh282)
安徽医科大学科研基金项目(2019xkj062)
出版年
2024
国家科技期刊平台
NSTL
万方数据