MiR-19a affects hemangioma stem cells proliferation and migration by targeting insulin-like growth factor 2 receptor
Objective To investigate whether miR-19a interacts with insulin-like growth factor 2 receptors(IGF-2R)in infantile hemangiomas(IHs)and affects the proliferation,migration,and adipogenesis of hemangioma stem cells(HemSCs).Methods HemSCs were isolated,screened and cultured from IH specimens.IGF-2R expression in HemSCs was identified using immunohistochemistry.HemSCs transfected with miR-19a mimics and inhibitors were subjected to CCK-8,wound healing,Transwell,qRT-PCR,and Western blot analyses.Results Compared with the control,the proliferation and migration rate of HemSCs treated with miR-19a inhibitors were significantly increased,and overexpression of miR-19a significantly inhibited IGF-2 induced cell migration and proliferation(P＜0.05).Conclusion MiR-19a may inhibit HemSCs proliferation,migration,and adipogenesis by targeting IGF-2R.
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