首页|两样本孟德尔随机化探讨欧洲类风湿关节炎人群与缺铁性贫血的因果关系

两样本孟德尔随机化探讨欧洲类风湿关节炎人群与缺铁性贫血的因果关系

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的 通过两样本孟德尔随机化法探讨欧洲类风湿关节炎(RA)人群与缺铁性贫血(IDA)的因果关系。方法 RA与IDA的单核苷酸多态性(SNPs)均使用公开的全基因组关联研究(GWAS)数据进行汇总分析。使用逆方差加权法(IVW)作为主要分析方法评估RA对IDA的因果效应。以MR-Egger回归法、加权中位数法(WM)、加权模式和简单模式作为回归补充说明，进而评估敏感性分析结果的稳健性。用Heterogeneity函数计算P值检验异质性、用截距项Intercept检验水平多效性。结果 在全基因组水平FINNGEN数据库中选择出了经孟德尔随机分析去除连锁不平衡且满足P＜5。0 ×10-8的强相关SNPs，整合暴露与结局数据后获得31个SNPs作为最终的有效工具变量。IVW法显示RA是IDA的风险因素(在RA的患病人群中，发生IDA的风险是无 RA 人群的 1。064 倍，OR=1。064，95％CI:1。028～1。103);WM和MR-Egger回归结果支持RA对IDA存在正向关联;截距项数值趋近于0，表明暴露与结局不存在水平多效性;Heterogeneity函数P＜0。05，表明暴露与结局存在异质性，但经随机效应模型检验，P＜0。05，表明因果关系即使存在异质性，但总体趋于稳健。结论 RA是IDA的危险因素，RA与IDA存在正向关联。
To explore the causal relationship between rheumatoid arthritis and iron deficiency anemia in European population by two-sample Mendelian randomization
Objective To explore the causal relationship between rheumatoid arthritis(RA)and iron deficiency a-nemia(IDA)in European population by two-sample Mendelian randomization analysis.Methods The single nu-cleotide polymorphisms(SNPs)of RA and IDA were analyzed using public genome-wide association studies(GWAS).The inverse variance weighting method(IVW)was used as the main analysis method to evaluate the causal effect of RA on IDA.MR-Egger method,weighted median method(WM),weighted model method and simple model method were used as regression supplements to evaluate the robustness of sensitivity analysis results.The het-erogeneity function was used to calculate the P-value to test the heterogeneity,and the intercept term intercept was used to test the level pleiotropy.Results In the FINNGEN database at the genome-wide level,strong-related SNPs that removed linkage disequilibrium and met the P＜5.0 × 10-8 by Mendelian randomization analysis were select-ed.After integrating exposure and outcome data,31 SNPs were obtained as the final effective instrumental variables.IVW showed that RA was a risk factor for IDA(the risk of IDA in RA patients was 1.064 times higher than that in non-RA patients,OR=1.064,95％CI:1.028-1.103).The weighted median method and MR-Egger method re-sults supported the positive correlation between RA and IDA.The intercept value was close to 0,indicating that there was no horizontal pleiotropy between exposure and outcome.The heterogeneity function's P＜0.05 indicated that there was heterogeneity between exposure and outcome,but the random effect model test showed P＜0.05,indi-cating that even if there was heterogeneity in causality,the overall trend was stable.Conclusion RA is a risk factor for IDA,and there is a positive correlation between RA and IDA.

rheumatoid arthritisiron deficiency anemiatwo-sample mendelian randomizationcausal association

王勇、姚晓玲、杨豫正、凌益、姚血明、马武开

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贵州中医药大学第二附属医院风湿免疫科,贵阳 550001

类风湿关节炎 缺铁性贫血 两样本孟德尔随机化 因果关联

国家自然科学基金贵州省中医风湿免疫病临床研究中心中医药、民族医药科学技术研究专项课题项目贵州中医药大学国家与省级科技创新人才团队培育项目

82274678黔科合平台人才[2020]2202号QZYY-2023-020贵中医TD合字[2022]004号

2024

10.19405/j.cnki.issn1000-1492.2024.07.022

安徽医科大学学报
安徽医科大学

安徽医科大学学报

CSTPCD北大核心
影响因子：1.095
ISSN：1000-1492
年,卷(期)：2024.59(7)
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