MicroRNA-141-5p/ABCG1 reverses imatinib resistance in K562 chronic myeloid leukemia cells
Objective To explore the mechanism of miR-141-5p and its effect on Imatinib(IM)resistance in CML.Methods qRT-PCR was used to detect miR-141-5p mRNA levels in IM resistant and sensitive patients.Western blot was used to detect the expression of proteins such as MMP-3,MMP-9,and Bcl-2 before and after transfection in K562 and K562/G01 cells.CCK-8 was used to detect of K562 and K562/G01 cell activity;Flow cytometry assay was used to detect the binding of miR-141-5p with ABCG1;Nude mice were used to validate the effect of miR-141-5p on tumors in vivo.Results The results showed that miR-141-5p was downregulated in IM-resistant CML patients and IM-resistant CML cells and overexpression of miR-141-5p could inhibit the growth of IM-resistant CML cells and promote their apoptosis.Research on tumor bearing mice had shown that miR-141-5p in-hibits tumor growth in vivo.Finally,it was found that miR-141-5p could directly target ABCG1 in IM-resistant CML cells to regulate CML occurrence.Conclusion miR-141-5p and ABCG1 form a competing endogenous RNA(ceRNA)network to function in IM resistance,thus facilitating CML progression.
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