The absence of GPR108 results in decreased inflammatory response in lipopolysaccharide-induced sepsis mice
Objective To investigate the effect of G protein-coupled receptor 108(GPR108)gene knockout on systemic inflammation in lipopolysaccharide(LPS)-induced sepsis mice.Methods Male C57BL/6 mice and GPR108 gene knockout mice were randomly divided into 4 groups:WT group,WT-LPS group,KO group,KO-LPS group.The physiological characteristics of mice in different groups were observed,and the morphological changes of liver and lung tissues were observed.Macrophages were extracted from bone marrow and subjected to flow cytometry to detect their M1 polarization status.The expression levels of IL-6 in liver and lung tissues,macrophages,and ser-um were also measured.Results KO-LPS group mice showed significant liver and lung tissue damage,with a sig-nificantly greater number of bone marrow-derived macrophages polarizing towards M1 in the KO-LPS group com-pared to the WT-LPS group.Additionally,at the tissue,cellular,and serum levels,the expression of IL-6 in the KO-LPS group mice was significantly higher than that in the WT-LPS group mice(P＜0.05).Conclusion Dur-ing the systemic inflammatory infection induced by LPS in mice,the lack of GPR108 exacerbates the systemic in-flammatory response.GPR108 has an inhibitory effect on the inflammatory response in mice with LPS-induced sep-sis.
万方数据
维普
