Pancreatic stellate cells promote the PIK3C2A expression and growth of pancreatic cancer cells through paracrine effects
Objective To study the effect and regulatory mechanism of secreted proteins from PSC on pancreatic ductal adenocarcinoma cells(PANC-1).Methods Conditioned medium(CM)from pancreatic stellate cells(PSC)was collected through an indirect co-culture method,and PANC-1 cells were cultured separately with CM for 0,2,and 24 h.The proliferation phenotype of PANC-1 cells under different stimulation periods was detected u-sing the CCK-8 assay.Proteomic analysis was performed to analyze the changes in protein levels of PANC-1 cells,and the most significant protein changes were validated using Western blot.Results Compared with the control group,the proliferation rate of PANC-1 cells increased after being stimulated by PSC derived CM;The results of proteomic analysis showed that the protein expression of metabolic pathways in PANC-1 cells increased continuously after being cultured in PSC CM for 0,2,and 24 h.Western blot analysis confirmed an increasing trend of PIK3C2A in PANC-1 cells,indicating that the CM from PSC might promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A.Conclusion The CM of PSC may promote the proliferation of PANC-1 cells by upregulating the expression of PIK3C2A,which improves the understanding of the mechanism of interaction between PSCs and pancreatic cancer cells in the tumor microenvironment.
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