M1 polarization of macrophage induced by STING signaling promotes T cell immune response
Objective To investigate the effect of activation of the stimulator of interferon genes(STING)pathway on macrophage polarization function and its role in T-cell response.Methods Mouse macrophage RAW264.7 cells were used.STING signaling related proteins in RAW264.7 macrophage treated with STING agonist diABZI were analyzed by Western blot,including TANK-binding kinase-l(TBK1),interferon regulatory factor-3(IRF3),STING,p-TBK1,p-IRF3,p-STING.The polarization of macrophage RAW264.7 cells treated with diABZI was an-alyzed by flow cytometry.Co-culture of diABZI-treated RAW264.7 macrophage and T cells was applied to evaluate the change of T cell response.Results STING signaling related proteins were upregulated in macrophage RAW264.7 cells treated with diABZI for 3 hours.The expression of CD86 was upregulated on the surface of macro-phages after 12 hours of diABZI treatment,and the CD86/CD206 ratio was elevated,which presented the M1 po-larization phenotype.When coculturing diABZI-treated macrophage RAW264.7 cells with T cells,the cytokine se-cretion ability of T cells including CD4+T and CD8+T cells was enhanced and the expression of CD107a in CD8+T cells was upregulated.Conclusion STING signaling induces M1 polarization of macrophages which enhance the function of T cells,especially CD8+T cell immune response.
macrophageSTING signalingpolarization of macrophageT cell immune responseCD4+T cellsCD8+T cells
万方数据
维普
