The Study of Mechanism of Bushen Yigu Recipe on Regulating Bone Metabolism to Treat Osteoporosis Based on "Balance" Theory
Objective:To explore the anti-osteoporosis mechanism of Bushen Yigu prescription by regulating bone metabolism and bone transformation balance in osteoporosis rat model based on"balance theory".Methods:60 8-month-old SPF SD rats were randomly divided into blank group,model group,control group,high,medium and low dose groups(n=10).All rats underwent bilateral ovarian removal surgery to create a model.The control group was intra-gastrically administered with sodium alendronate for 2 weeks,the model group was intragastrically administered with an equal amount of normal saline,and the high,medium,and low-dose groups were administered different doses according to the group.The kidney-tonifying and bone-reinforcing prescription liquid is administered into the stomach.The drug intervention lasted for 12 weeks.HE was taken from the middle part of the femoral bone of rats to detect the pathologi-cal changes of the femur.The venous blood of the rats was collected to detect the blood Ca and P concentrations in the serum.The expression levels of PINP,PICP,CTX and NTX were detected by ELISA.Results:H staining examina-tion showed that the bone tissue in the model group showed obvious osteoporotic changes.The pathological changes of osteoporosis in rats were significantly improved after drug intervention;the blood phosphorus and blood calcium con-centrations of rats in the model group were lower than those of the blank group(P＜0.05),and the blood phosphorus and blood calcium concentrations increased after drug intervention(P＞0.05);The concentrations of PINP,PICP,CTX and NTX in rats in the model group were significantly higher than those in the blank group(P＜0.05),while the concentra-tions of PINP,PICP,CTX and NTX in the control group and low,medium and high dose groups were lower than those in the model group.(P＜0.05);the concentrations of PINP,PICP,CTX and NTX in rats in the low,medium and high dose groups were lower than those in the control group(P＜0.05).There was no statistical difference between groups(P＞0.05).Conclusion:Bushen Yigu Recipe can improve bone quality in ovariectomized osteoporosis rat models.The mechanism is related to regulating calcium and phosphorus metabolism in rats,curbing excessive bone metabolism,and reducing bone turnover rate.Different doses of"Bushen Yigu Recipe"are useless.There is a dose-dependent trend,and the ef-fect of regulating bone metabolism is obvious at moderate doses.
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