Study on the Mechanism of Achyranthes Bidentata-Cuscutae Medicine in Preventing and Treating Hypertensive Renal Injury based on Network Pharmacology
Objective: To explore the potential mechanism of action of Achyranthes bidentata-Cuscutae in the treat-ment of hypertensive renal injury by using network pharmacology method. Methods: TCMSP and Uniprot databases were used to obtain the active ingredients and target genes of Achyranthes bidentata and Cuscuta japonica respectively. The disease-related targets of hypertensive renal injury were obtained through GenenCards and Omim databases. Obtain the intersection genes of drugs and diseases in the Venny online tool, and use the STRING database and Cytoscape 3.9.1 software to construct a protein interaction network. Construct GO and KEGG pathway enrichment analysis through the data obtained from the Metascape database and the Weishengxin website, and establish an active ingredient-target-key pathway network. Results: In the TCMSP database, a total of 24 active pharmaceutical ingredients for Achyranthes bidentata and Cuscuta japonica were screened out, and the number of active ingredient targets and disease targets were 206 and 1762 respectively. 113 genes common to drugs and diseases were obtained in the Venny online tool. The PPI network shows that Achyranthes bidentata and Cuscutae drugs play an important role in the following targets: mitogen-activated protein kinase 1, tumor suppressor protein P53, protein kinase B1, tumor necrosis factor, proto-oncogene,interleukin-6, etc. From the GO and KEGG pathway enrichment analysis results, it can be seen that the intersection genes of drugs and diseases are closely related to molecular biological processes such as inorganic substances, oxidative stress, chemical stress, reactive oxygen species, and metal ions. Related signaling pathways include: cancer pathways, lipids and atherosclerosis, AGE signaling pathways in diabetic complications, etc. Conclusion: Achyranthes bidentata-Cuscutae has basic pharmacological activity in treating hypertensive renal injury, and may improve hypertensive renal injury by acting on tumor suppressor protein P53, protein kinase B1 and other targets through the AGE-RAGE signal-ing pathway and tumor necrosis factor signaling pathway.
Hypertensive renal damageTraditional Chinese medicine pharmacologyAchyranthes bidentataCuscuta semen
万方数据
维普
