Intervention Effect of Tongxinluo Capsules on Myocardial Fibrosis in Spontaneously Hypertensive Rats
Objective: To observe the intervention effect and mechanism of Tongxinluo Capsule on myocardial fibro-sis in spontaneously hypertensive rats (SHR). Methods: A total of 408-week-old male SHR rats were randomly divided into high-, medium-, and low-dose Tongxinluo treatment groups and a positive control group, with 10 rats in each group; 108-week-old Wistar rats served as the normal control group. After 12 weeks of oral administration in each group, myocardial fibrosis was detected by Masson's method, α-SMA and TGF mRNA expression were detected by real-time reverse transcription PCR, and phosphorylated Smad3 and CTGF protein expression were detected by West-ern blotting. Results: Tongxinluo capsule alleviated SHR myocardial fibrosis, inhibited α-SMA mRNA expression, reduced the phosphorylation level of Smad3 and CTGF expression in SHR hearts, but did not change the expression of TGFβ1 mRNA. Conclusion: Tongxinluo Capsule inhibits the transformation of cardiac fibroblasts and improves myo-cardial fibrosis in hypertension by inhibiting the TGFβ1-independent Smad3/CTGF pro-fibrotic pathway.
万方数据
维普
