Effect of Epimedium Flavonoids on Tartaric Acid Phosphatase 5b in Ovariectomized Rats and its Correlation with Bone Mineral Density
Objective: To explore the effect of epimedium total flavonoids (TFE) on tartrate-resistant acid phos-phatase 5b (TRACP-5b) and bone mineral density in ovariectomized rats. Methods: 10-month-old female SD rats were ovariectomized to establish a rat model. The random number table method was divided into blank control group (N), model control group (O), TFE low (TL), medium (TM), and high dose. Group (TH) and β-estradiol group (E), 10 ani-mals each, were administered intragastric intervention for 90 days. The tartrate-resistant acid phosphatase kit was used to determine the TRACP5b content, and the small animal analysis software was used to determine the whole body bone mineral density in vivo. Results:① The level of TRACP-5b in group O was significantly higher than that in group N, with a significant difference (P<0.01). The levels of TRACP-5b in groups TL, TM, TH and E were significantly lower than that in group O, with a significant difference (P<0.01). ② Compared with group N, the bone density of lumbar vertebrae in groups O, TL, TM, TH and E all decreased, and only group O showed a significant difference (P<0.05);compared with group O, the bone density of lumbar vertebrae in groups TM and TH decreased. The bone density of the lumbar vertebrae in group E increased with a significant difference (P<0.05). The bone density of the lumbar spine in group E increased with a significant difference (P<0.01). ③ Compared with the N group, the whole body bone density of the O, TL, TM, TH and E groups all decreased, and only the difference in the O group was statistically significant (P<0.05); compared with the O group, the TL, TM, TH The whole body bone density of group E and group E increased, but there was a significant difference only in group E (P<0.05). Conclusion: TFE can reduce the content of TRACP-5b in the serum of ovariectomized rats, and has a certain dose-effect relationship with the lumbar spine bone density and whole body bone density of ovariectomized rats.
Epimedium total flavonoidsCastrated ratsTartrate-resistant acid phosphatase 5bBone density
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