Discussion on Comorbidity Mechanism of Alzheimer's Disease and Osteoporosis and Prediction of Potential Chinese Medicine based on GEO Database
Objective:Use bioinformatics methods to obtain the differential genes and related pathways between Alz-heimer Disease(AD)and osteoporosis(OP),and predict them.Methods:Through the gene chip data in the GEO database,taking AD-OP as the research object,the differential genes and protein interaction networks of AD-OP patients were screened out,and the CytoHubba plug-in was used to identify Hub genes.The MCODE plug-in pair was closely related to the onset of AD-OP.Cluster analysis was performed on related functional modules,and the TRRUST database was used to predict transcription factors of common differentially expressed genes.Through GO function and KEGG pathway,its mechanism of action was clarified,and the differentially expressed genes in the validation group were used to verify the above research results.Finally,the Coremine Medical database was used to predict traditional Chinese medicines that have significant regulatory effects on key genes.Results:31 AD-OP differentially expressed genes were obtained from the GEO database,and imported into the STRING database for retrieval,27 nodes and 54 edge groups were obtained.The MCODE plug-in performs cluster analysis and mines 2 disease-related functional modules.The CytoHubba plug-in finds 25 hub genes in the associated network data and uploads them to the GeneMANIA database.It is concluded that ACTR1A,DCTN1,ARPC1B,CTTN,etc.are most closely related to the core genes.genes,and then use GO annotation and KEGG pathways to analyze the above genes.The enrichment analysis results showed that pathways of multiple neurodegenerative diseases and the p53 signaling pathway are crucial to the pathogenesis of AD-OP.Through TRRUST analysis,three major transcription factors including TP53,RELA,and NFKB1 were obtained,as well as genes regulated by transcription factors such as MGP,CARM1,BCL2L1,BCL2,AXIN2,ACACA,UBE2C,PTP4A1,and PTEN.COREMINE was used to pre-dict key genes and pathways,and the results showed that comfrey,turmeric,turmeric,and polygonatum are core Chinese medicines in the treatment of AD-OP.Conclusion:This study clarified the core genes of AD-OP and the gene-enriched pathways,explored the biological processes mainly involved in AD-OP,and used the validation data set to verify the Hub gene as a new target for AD-OP treatment.Point selection provides support and predicts therapeutic Chinese medicines targeting core genes,providing a basis for treatment and research on AD-OP.
ADOPGEO databaseComorbidity mechanismTraditional Chinese medicine
国家科技期刊平台
NSTL
万方数据
