Study on the Mechanism of Tanreqing Injection in Treating Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Objective:To study the mechanism of Tanreqing injection in the treatment of acute exacerbation of chronic obstructive pulmonary disease.Methods:Online query of the TCMSP database and previous literature to ob-tain the main active ingredients of traditional Chinese medicines such as skullcap,bear bile powder,goat horn,hon-eysuckle,and forsythia suspensa,and use the swisstargetprediction database to obtain the targets corresponding to the active ingredients;use GeneCards,OMMI,Drug Bank and other databases Obtain targets for acute exacerbation of chronic obstructive pulmonary disease.Use the Venny platform to obtain the intersection of drug and disease targets online,use the DAVID database to conduct gene ontology analysis and pathway enrichment analysis of the intersecting targets,use the STRING database to construct a protein interaction network,and use the Cytoscapr software to visualize and screen the main active ingredients,Key targets.Finally,the core targets and core components were verified by mo-lecular docking through AutoDock vina software,and pymol was used for visual processing.Results:Tanreqing injec-tion contains 90 main active ingredients,corresponding to 557 targets;Forsythia suspensa,skullcap,and honeysuckle are the key drugs of Tanreqing injection;baicalein,Norwogonin,5,7,4'-Trihydroxy-8-methoxyflavone,5,2',6'-Tri-hydroxy-7,8-dimethoxyflavone,(2R)-7-hydroxy-5-methoxy-2-phenylchroman-4-one,Panicolin,SkullcapflavoneⅡ,Moslosooflavone,5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone,quercetin,etc.are core active ingredients;STAT3,HSP90AA1,ESR1,SRC,EP300,AKT1,JUN,PIK3R1,RELA and other targets are core targets in the protein interac-tion network point.Conclusion:Tanreqing injection therapy mainly plays a potential role in the acute exacerbation of chronic obstructive pulmonary disease by regulating cellular inflammatory response,cell proliferation,and apoptosis through flavonoids.
Tanreqing injectionAcute exacerbation of chronic obstructive pulmonary diseaseNetwork pharmacol-ogyMolecular docking technologyMechanism of action
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