Network Pharmacology Study of Chiyin Mixture Mediating Mitogen-activated Protein Kinase-related Pathway to Improve CHB Liver Fibrosis
Objective:Based on network pharmacology and molecular docking technology,to explore the relevant mechanism of action of Chiyin Mixture,an in-hospital preparation of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,in the treatment of chronic hepatitis B liver fibrosis.Methods:The Traditional Chinese Medicine Systems Pharmacology Analysis Platform(TCMSP)was used to search and download the potentially effective active ingredients of each medicine in Chiyin Mixture and the corresponding targets in the human body,and search and download from Genecards,OMIM,DrugBank,PharmGkb,and TTD databases.For the targets of chronic hepatitis B liv-er fibrosis,use Venn online mapping software to obtain common targets of drugs and diseases and draw Venn diagrams,use String database to establish protein interaction(PPI)networks between common targets,and use Cytoscape The CytoNCA plug-in in the software obtains the key targets in the PPI network,uses the R package to perform GO func-tional enrichment analysis on the intersection targets,and obtains the KEGG pathway enrichment analysis results in the same way.Use Cytoscape software to construct a"drug-disease-target-pathway"network diagram,obtain key ac-tive ingredients by analyzing the"drug-disease-target-pathway"network,and use AutoDock Vina software to compare the screened key active ingredients of Chiyin Mixture with Disease-key drug targets are simulated molecular docking one by one.Results:A total of 202 compounds of the Chiyin mixture were screened out,with 298 effective targets.282 drug-disease common targets were obtained through the Venn diagram,and the PPI network found that JUN,MAPK3,TP53,AKT1,MAPK1,MAPK14,etc.may be the key targets of Chiyin Mixture in the treatment of chronic hepatitis B liver fibrosis.GO analysis contained a total of 3120 enrichment results,and KEGG pathway analysis found 191 pathway enrichment results.Molecular docking results show that Chiyin Mixture may intervene in the activities of chronic hepa-titis B liver fibrosis through the TP53,MAPK3,and MAPK14 pathways to achieve therapeutic effects.Conclusion:Chi-yin mixture plays a role in treating CHB liver fibrosis through"multi-component-multi-target-multi-pathway".The 10 active ingredients in the red yeast mixture,including quercetin,daidzein,naringenin,baicalein,emodin,luteolin,puerarin,tanshinol,and tanshinone ⅡA,deserve the focus of subsequent research.Through multi-faceted integrated analysis of the full text,it is speculated that Chiyin Mixture plays a role in improving CHB liver fibrosis by mediating mitogen-activated protein kinase through the PI3K/AKT pathwav.
Chiyin mixtureChronic hepatitis BLiver fibrosisNetwork pharmacologyMolecular docking
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