摘要
目的:探究地榆槐花汤通过沉默KRT17基因对人结肠癌细胞活力、迁移侵袭能力和糖酵解的影响.方法:用空白对照组和低、中、高(10％、15％、20％)体积分数(V/V)地榆槐花汤含药血清作用于体外培养的人结肠癌HCT116细胞,CCK8法检测细胞的活力,Transwell检测迁移和侵袭,糖酵解试剂盒检测葡萄糖及乳酸水平;通过GEO、GEPIA数据库筛选结肠癌中的差异表达基因KRT17,检测地榆槐花汤对KRT17的影响及KRT17对结肠癌细胞活力、迁移、侵袭和糖酵解水平的影响;检测地榆槐花汤/KRT17轴对细胞的活力、迁移、侵袭及糖酵解水平的影响;结果:地榆槐花汤可抑制结肠癌细胞活力、迁移、侵袭及糖酵解水平(P＜0.05),且呈剂量依赖性;KRT17基因在结肠癌中高表达并可以促进结肠癌细胞活力、迁移、侵袭及糖酵解水平(P＜0.05);地榆槐花汤可以抑制KRT17的表达(P＜0.05),且过表达KRT17可以逆转地榆槐花汤对结肠癌细胞活力、迁移、侵袭及糖酵解的抑制作用(P＜0.05).结论:地榆槐花汤可以通过调控KRT17基因表达抑制结肠癌细胞活力、迁移、侵袭,促进糖酵解.
Abstract
AIM:To explore the effects of Diyuhuaihua Decoction on human colon cancer cell viability,invasion and migration ability and glycolysis by silencing KRT17 gene expression METHODS:Human colon cancer HCT116 cells cultured in vitro were treated with a blank control group and low,medium and high(10％,15％,20％)vol-ume serum containing Diyuwuhua decoction.The cell viability was detected by CCK8 method,migration and invasion were detected by Transwell,glucose and lactic acid levels were detected by glycolysis kit.The differentially expressed gene KRT17 in colon cancer was screened by GEO and GEPIA databases,to detect the effect of Diyuhuahua decoc-tion on KRT17 and effect of KRT17 on viability,migration,invasion and glycolysis of colon cancer cells.The effects of Diyuhuaihua Decoction/KRT17 axis on cell viability,migration,invasion and glycolysis were detected.RESULTS:Diyuhuaihua decoction can inhibit the activity,migration,invasion and glycolysis of colon cancer cells(P＜0.05).The KRT17 gene is highly expressed in colon cancer and can promote the viability,migration,invasion and glycolysis of colon cancer cells(P＜0.05).Diyuhuaihua decoction can inhibit the expression of KRT17(P＜0.05),and overex-pression of KRT17 can reverse the inhibitory effect of Diyuhuaihua decoction on the viability,migration,invasion and glycolysis of colon cancer cells(P＜0.05).CONCLUSION:Diyuhuaihua decoction can inhibit the viability,migration and invasion of colon cancer cells and promote glycolysis by regulating the expression of KRT17 gene
基金项目
2021年度黑龙江省省属高等学校基本科研项目(2021-KYYWF-0370)
国家科技期刊平台
NSTL
万方数据