Study on the Effect and Mechanism of Rhodiola Rosea on Neurological Function after Acute Cerebral Hemorrhage
Objective:To observe the effect of Rhodiola rosea on the neurological function of rats with acute cer-ebral hemorrhage(ACH)and to explore its mechanism of action.Methods:The experimental rats were randomly divided into sham operation group(Sham group),acute cerebral hemorrhage group(ACH group)and Rhodiola rosea group.The Bederson score of neurological function and behavioral evaluation were performed on the rats in each group,and the cell ultra-structure was observed by transmission electron microscopy(TEM).The expression of interleukin-1 β(IL-1 β),tumor necrosis factor-α(TNF-α)and transforming growth factor-β(TGF-β)was detected by ELISA,and the expression of CD 16 and CD206 was detected by immunofluorescence.The contents of glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and iron(Fe)in each group were determined.Results:After treatment with Rhodiola rosea,the neurological deficit score of ACH rats was significantly reduced,and the behavioral evaluations such as drinking,eating,and activity were significantly improved,and the mortality rate decreased,with statistically significant differ-ences(P＜0.05).TEM observation showed that the tissue around the hematoma in the ACH group was loose,the nuclear membrane structure was unclear,the organelles were reduced,the mitochondria were swollen,the number was reduced,and vacuolar changes occurred.After treatment with Rhodiola rosea,the cell swelling was reduced and the structure remained relatively intact.After modeling,the expression of GSH-PX in the ACH group decreased,the expression of MDA increased,and the content of Fe increased.After treatment with Rhodiola rosea,the expression of GSH-PX in-creased,and the contents of MDA and Fe decreased.ELISA results showed that compared with the Sham group,the ex-pression of IL-1 β and TNF-α in the ACH group was significantly increased(P＜0.05);compared with the ACH group,the Rhodiola rosea group significantly downregulated the expression of IL-1 β and TNF-α,and up-regulated the ex-pression of TGF-β(P＜0.05);the CD16 fluorescence expression in the ACH group was higher than that in the Rhodiola rosea group,and the CD206 was lower than that in the Rhodiola rosea group.Conclusion:Rhodiola rosea has a good therapeutic effect on acute cerebral hemorrhage,and its mechanism may be related to inhibiting inflammatory response,reducing oxidative stress,alleviating ferroptosis and promoting micro-glial polarization.
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