Network Pharmacology Study on the Prevention and Treatment of Gastric Cancer Precancerous Lesions by Si Ni San
Objective:To gain a deeper understanding of the material basis and possible mechanism of the treatment of gastric precancerous lesions(PLGC)with Sinisan,a prescription in Treatise on Febrile Diseases,through network pharmacology.Methods:The effective active ingredients and their corresponding targets in Sinisan were searched and screened using the Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP)database,and the targets related to PLGC were explored by combining GeneCard and OMIM databases.EXCEL software was used to ob-tain the common targets of the two,and then Cytoscape software was used to draw a network diagram of"Sinisan active ingredients-intersection targets-gastric precancerous lesions";the String platform was used to obtain the network diagram of protein interactions,and the Cytoscape software was used to screen out the core targets and visualize them into a network diagram of"Sinisan key targets for the treatment of PLGC";David platform was used to perform gene ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis on the obtained targets.Results:It was predicted that there were 118 active ingredients in Sini San for the treatment of PLGC,cor-responding to 808 targets,2362 disease targets,and 298 targets intersecting Sini San and PLGC.53 key targets were screened out from the PPI core network using the median values of BC,CC,and DC,and further GO functional enrich-ment and KEGG pathway enrichment analysis were performed.GO functional enrichment obtained 1062 biological processes,which were mainly closely related to cell growth and proliferation,apoptosis transformation,and gene tran-scription regulation biological processes.There were 298 KEGG enriched pathways,and the top 20 were screened out,showing that they were significantly enriched in cancer pathways,epidermal growth factor signaling pathways,CKAP4 signaling pathways,gastrin signaling pathways,etc.Conclusion:Sinisan can treat PLGC by regulating cancer pathways,epidermal growth factor signaling pathways,CKAP4 signaling pathways,gastrin signaling pathways,etc.through flavo-noids such as calycosin,naringenin,and galangin,thereby improving gastric microenvironment,reducing mucosal in-flammatory damage,and inhibiting the malignant growth of cells to treat PLGC.This provides a certain reference for the clinical application of traditional Chinese medicine prescriptions to treat PLGC.
万方数据
维普
