Structure and Anti-inflammatory Activity of Two Sanghuangporus vaninii Polysaccharides
Objective To isolate and purify polysaccharides from Sanghuangporus vaninii,and to investigate their structural features and in vitro anti-inflammatory activity.Methods The water-extraction and alcohol-precipitation method was used to obtain Sanghuang polysaccharide(SHP),and the Cellulose DE-52 and Sephadex G-100 columns were used for isolation and purification.The structure of the polysaccharides were analyzed using gel permeation chromatography-refractive index-multi an-gle laser scattering,ion chromatography,Fourier transform infrared spectroscopy,and scanning electron microscopy,and ELISA was used to observe their influence on the release of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β).Results Two homogeneous acidic polysaccharides,SHP-1-1 and SHP-2-1,were isolated and purified from Sanghuangporus vaninii.SHP-1-1 had a weight-average molecular weight of 333.599 kDa and was composed of fucose,arabinose,rhamnose,galactose,glucose,xylose,mannose,and glucuronic acid at a molar ratio of 11.48∶0.18∶0.28∶17.86∶27.71∶0.64∶11.75∶0.94;SHP-2-1 had a weight-average molecular weight of 563.032 kDa and was composed of fucose,arabi-nose,rhamnose,galactose,glucose,xylose,mannose,and glucuronic acid at a molar ratio of 0.73∶0.14∶0.32∶1.13∶14.96∶1.04∶3.79∶1.50.Both SHP-1-1 and SHP-2-1 could reduce the levels of the inflammatory factors NO,TNF-α,IL-6,and IL-1β induced by lipopolysaccharide in RAW264.7 cells,with a half-maximal inhibitory concentration of 32.35-96.31 μg/mL.Conclusion SHP-1-1 and SHP-2-1 are homogeneous polysaccharides with anti-inflammatory activity,and the research on their structure and activity provides a certain reference for the development and utilization of Sanghuangporus vaninii.
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