Effect of Moxibustion on Behavior and c-fos in the Lateral Hypothalamic Region of Mice with Posttraumatic Stress Disorder
Objective To investigate the effect of moxibustion at Neiguan and Yanglingquan on the behavior of mice with posttraumatic stress disorder (PTSD)and the expression of c-fos in the lateral hypothalamic region.Methods C57 mice were randomly divided into normal group,model group,and moxibustion group,with 6 mice in each group.The mice in the model group and the moxibustion group were given modified single prolonged stress and electrical stimulation to establish a model of PTSD,and the mice in the moxibustion group were given moxibustion at Neiguan and Yanglingquan for 7 consecutive days.The open field test,elevated plus maze,and fear conditioning task were used to observe the behavior of mice,and immunofluorescence assay was used to measure the expression of c-fos in the lateral hypothalamic region of mice.Results Compared with the normal group,the model group had significant reductions in the time spent and the distance traveled in the center of the open field test (P<0.05)and the number of entries and time in the open arms of the elevated plus maze test (P<0.05),as well as significant increases in the freezing time of background fear test and acoustic fear test in fear conditioning task (P<0.05),and compared with the normal group,the model group had a significant increase in the expression level of c-fos in the lateral hypothalamic region (P<0.05).Compared with the model group,the moxibustion group had significant increases in the time spent and the distance traveled in the center of the open field test (P<0.05)and the number of entries and time in the open arms of the elevated plus maze test (P<0.05),as well as significant reductions in the freezing time of background fear test and acoustic fear test in fear conditioning task (P<0.05),and compared with the model group,the moxibustion group had a significant reduction in the expres-sion level of c-fos in the lateral hypothalamic region (P<0.05).Conclusion Moxibustion at Neiguan and Yanglingquan can significantly improve anxiety-and depression-like behavior in PTSD mice,possibly by acting on c-fos in the lateral hypothalamic region of mice.
Posttraumatic stress disorderMoxibustionc-fosLateral hypothalamic region
万方数据
维普
