Mechanism of Pingchuanning in Intervention of Airway Inflammation in Rats with Cold-type Asthma by Regulating the Transient Receptor Potential Melastatin Subtype 8 Channel-related Proteins
Objective To investigate the effect of cold stimulation on airway inflammation in asthmatic rats,as well as the ther-apeutic effect of Pingchuanning on airway inflammation in rats with cold-type asthma and its mechanism.Methods A total of 72 rats were randomly divided into normal group,ovalbumin (OVA)group,model group (OVA+cold stimulation),Guilong Kechuanning group,dexamethasone group,and low-,middle-,and high-dose Pingchuanning groups,with 9 rats in each group.All rats except those in the normal group were given OVA or OVA combined with cold stimulation to establish a model of asthma or cold-type asthma,and after modeling,the rats were given normal saline or the corresponding drug solution by ga-vage for 21 days.General behavioral manifestations were recorded for each group;HE staining was used to observe the patho-logical changes of lung tissue;ELISA was used to measure the levels of the inflammatory factors interleukin(IL)-1β,IL-6 and thymic stromal lymphopoietin (TSLP)in bronchoalveolar lavage fluid (BALF);Western blot was used to measure the expres-sion levels of transient receptor potential melastatin subtype 8 (TRPM8),granule macrophage-colony stimulating factor (GM-CSF)and TSLP.Results Compared with the normal group,the OVA group and the model group had the behavioral manifes-tations such as shortness of breath and obvious abdominal contraction;pathological results showed thickening of the bronchial wall and massive inflammatory cell infiltration in lung tissue and the peribronchial area;there were significant increases in the levels of IL-1β,IL-6,and TSLP in BALF (P<0.05)and the protein expression levels of TRPM8,GM-CSF,and TSLP in lung tissue (P<0.05).Compared with the model group,Pingchuanning improved the behavioral manifestation of rats,allevia-ted bronchial wall thickening and inflammatory cell infiltration in lung tissue and the peribronchial area,and significantly re-duced the levels of IL-1β,IL-6,and TSLP in BALF (P<0.05)and the protein expression levels of TRPM8,GM-CSF,and TSLP in lung tissue (P<0.05).Conclusion Cold stimulation aggravates airway inflammatory response in asthmatic rats,and Pingchuanning may exert a therapeutic effect on asthma by regulating the expression levels of TRPM8 and related proteins and alleviating airway inflammatory response in rats with cold-type asthma.
万方数据
维普
