Effect of Transnasal Administration of Agarwood Essential Oil on Posttraumatic Stress Disorder-like Behaviors and the Expression of GluN2A and GluN2B in the Hippocampus in Mice Induced by Single Prolonged Stress
Objective To investigate the effect of transnasal administration of agarwood essential oil on mice with posttraumatic stress disorder (PTSD)-like behaviors induced by single prolonged stress (SPS)and its possible mechanisms from the perspec-tive of synaptic plasticity.Methods Gas chromatography-mass spectrometry was used for qualitative analysis of the main com-ponents of agarwood essential oil.C57BL/6 mice were randomly divided into normal group,model group,low- and high-dose agarwood essential oil groups,and positive drug group,with 8 mice in each group.Behavioral experiments were used to assess the PTSD-like behaviors of SPS mice;the fear memory test was used to measure the fear freezing value of mice,and the open field test and the elevated plus maze test were used to assess anxiety-like behaviors;Western blot was used to measure the pro-tein expression levels of GluN2A and GluN2B in the hippocampus of mice.Results A total of 117 volatile components were de-tected in agarwood essential oil samples.Animal experiments showed that compared with the normal group,the model group showed marked anxiety-like behaviors and significant abnormalities in fear memory extinction (P<0.05),while agarwood es-sential oil and the positive drug ROL significantly improved the abnormal behaviors of SPS mice (P<0.05)and reversed the abnormal expression of GluN2A and GluN2B (P<0.05).Conclusion Transnasal administration of agarwood essential oil can improve the PTSD-like behaviors of mice induced by SPS,possibly by regulating the protein expression levels of GluN2A and GluN2B.
万方数据
维普
