摘要
目的 研究瓜蒌Trichosanthes kirilowii Maxim.干燥成熟果皮的化学成分.方法 应用硅胶、Sephadex LH-20凝胶、ODS反相色谱柱以及半制备HPLC等方法对瓜蒌皮水提物进行分离纯化,根据1 H-NMR、13 C-NMR和HRMS等波谱学方法对所得化合物进行结构鉴定.结果 从瓜蒌皮水提物的乙酸乙酯萃取部位共分离得到12个化合物,其结构鉴定为葫芦素D(1)、葫芦素B(2)、异葫芦素D(3)、fabacein(4)、23,24-二氢葫芦素D(5)、柯伊利素7-O-β-D吡喃葡萄糖苷(6)、blumenol A(7)、对羟基苯甲酸(8)、5-oxymaltol(9)、香草酸(10)、烟酸(11)和3-male-imide-5-oxime(12).结论 化合物4为首次从天然植物中分离得到,化合物3、8、9、11、12为首次从瓜蒌皮中分离得到.
Abstract
Objective To investigate the chemical components of the dry mature fruit peel of Trichosanthes kirilowii Maxim. Methods Silicagel,Sephadex LH-20,ODS reversed phase column,and semi-preparative HPLC were used for the isolation and purification of the water extract of Trichosanthes kirilowii Maxim.peel,and spectroscopy (1H-NMR,13C-NMR,and HRMS) was used for the structural identification of the compounds obtained.Results A total of 12 compounds were isolated from the ethyl acetate fraction of the water extract and were structurally identified as cucurbitacin D (1 ),cucurbitacin B (2 ),isocucurb-itacin D (3),fabacein (4),23,24-dihydrocu-curbitacin D (5),chrysoeriol-7-O-β-D-glucoside(6),blumenol A (7),4-hydroxy-benzoicacid (8 ), 5-oxymaltol (9 ), vanillic acid (10 ),nicotinic acid (11 ), and 2-methyl maleimide-5-oxime (12 ). Conclusion Compound 4 is isolated from a natural product for the first time,and compounds 3,8,9,11,and 12 are isolated from T.kirilowii peel for the first time.
基金项目
安徽中医药大学—潜山市医药健康发展产业科研攻关项目(2021QSHZ03)
安徽省高等学校自然科学研究项目(KJ2021A0594)
维普
万方数据