Effect of Electroacupuncture on Cerebral Blood Flow and Motor Function in Mice with Ischemic Stroke by Mediating the Adenosine Monophosphate-activated Protein Kinase/Glutamine-fructose-6-phosphate Aminotransferase 1/Vascular Endothelial Growth Factor Molec
Objective To investigate the effect of electroacupuncture at Baihui and Dazhui points on cerebral blood flow and motor function in mice with ischemic stroke,as well as the mechanism of action of the adenosine monophosphate-activated pro-tein kinase (MAPK )/glutamine-fructose-6-phosphate aminotransferase-1 (GFAT-1 )/vascular endothelial growth factor (VEGF)molecular pathway in electroacupuncture for alleviating vascular injury and improving limb movement.Methods Male C57 mice were randomly divided into sham-operation group,model group,and electroacupuncture group,and the method of pho-tothrombosis was used to establish a mouse model of cortical ischemia.The grid test and the cup-climbing test were used to e-valuate the motor function of mice;TTC staining and laser speckle flow imaging were used to evaluate the changes in cerebral infarct volume and blood perfusion;Western blot was used to measure the expression levels of AMPK,phosphorylated AMPK (p-AMPK),GFAT-1,and VEGF.Results Compared with the sham-operation group,the model group showed impaired mo-tor function (P<0.05),a significant increase in cerebral infarct volume (P<0.05),and a significant reduction in blood perfu-sion (P<0.05),as well as significant reductions in the protein expression levels of AMPK,p-AMPK,and VEGF (P<0.05) and a significant increase in the protein expression level of GFAT-1 (P<0.05).Compared with the model group,the electroacu-puncture group had a significant improvement in motor function(P<0.05),a significant reduction in cerebral infarct volume (P<0.05),and a significant increase in blood perfusion (P<0.05),as well as significant increases in the protein expression levels of AMPK,p-AMPK,and VEGF (P<0.05)and a significant reduction in the protein expression level of GFAT-1 (P<0.05).Conclusion Electroacupuncture can improve vascular injury and dyskinesia caused by cortical ischemia,possibly by me-diating the AMPK/GFAT-1/VEGF molecular pathway.
Ischemic strokeElectroacupunctureAdenosine monophosphate-activated protein kinaseGlutamine-fructose-6-phosphate aminotransferase 1Vascular endothelial growth factor
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维普
