Effect of Tongdu Tiaoshen Acupuncture Combined with Shibing Xingnao Granule on Gut Microbiota in Rats with Vascular Dementia
Objective To investigate the effect ofTongdu Tiaoshen acupuncture combined with Shibing Xingnao Granule ongut microbiota in rats with vascular dementia and its possible mechanism of action.Methods A total of 80 rats were randomly di-vided into sham-operation group,model group,Tongdu Tiaoshen acupuncture group,Shibing Xingnao Granule group,and Tongdu Tiaoshen acupuncture+Shibing Xingnao Granule group,with 10 rats in each group.The modified method of perma-nent ligation of both common carotid arteries was used to establish a model of vascular dementia.The rats in the Shibing Xing-nao Granule group were given the solution of Shibing Xingnao Granule by gavage,those in the Tongdu Tiaoshen acupuncture group were given Tongdu Tiaoshen acupuncture,and those in the Tongdu Tiaoshen acupuncture+Shibing Xingnao Granule group were given the solution of Tongdu Tiaoshen Acupuncture combined with Shibing Xingnao Granule.The Morris water maze test was used to evaluate the changes in cognitive and memory functions,and 16S rRNA sequencing was used to analyze the change in gut microbiota.Western blot was used to measure the protein expression levels of caspase-3,Bcl-2,and Bax. Results Compared with the sham-operation group,the model group had significant increases in mean escape latency(P<0.05) and the protein expression levels of caspase-3 and Bax (P<0.05),a significant reduction in the protein expression level of Bcl-2 (P<0.05),and a significant decrease in the relative abundance of probiotic bacteria in the intestine(P<0.05).Compared with the model group,the Tongdu Tiaoshen acupuncture group,the Shibing Xingnao Granule group,and the Tongdu Tiaoshen acupuncture+Shibing Xingnao Granule group had significant reductions in mean escape latency (P<0.05),the protein expres-sion levels of caspase-3,Bax (P<0.05),and the relative abundance of harmful intestinal bacteria (P<0.05),a significant in-crease in the protein expression level of Bcl-2(P<0.05).Conclusion Tongdu Tiaoshen acupuncture combined with Shibing Xingnao Granule can improve cognitive function in rats with vascular dementia,possibly by regulating the protein expression of caspase-3,Bax,and Bcl-2 in the hippocampus and affecting gut microbiota.
Tongdu Tiaoshen acupunctureShibing Xingnao GranuleGut microbiotaHippocampal CA1 region
万方数据
维普
