摘要
目的 观察电针"百会""大椎"对局灶性脑缺血大鼠酪氨酸蛋白激酶2/信号转导和转录激活因子3(Janus kinase 2/signal transducer and activator of transcription 3,JAK2/STAT3)通路和血管内皮生长因子(vascular endo-thelial growth factor,VEGF)的影响,探讨电针对局灶性脑缺血大鼠神经保护的作用机制.方法 将36只SD大鼠随机分为正常组、假手术组、模型组、电针组,每组9只,采用Longa线栓法进行局灶性脑缺血模型复制,电针组模型复制4 h后予以电针治疗,共治疗7 d.比较各组大鼠模型复制后4 h和7 d的神经功能评分;Western blot法检测各组大鼠脑组织JAK2、磷酸化酪氨酸蛋白激酶2(phosphorylated Janus kinase 2,p-JAK2)、STAT3、磷酸化信号转导和转录激活因子3(phosphorylated signal transducer and activator of transcription 3,p-STAT3)、VEGF蛋白表达水平;RT-PCR法检测各组大鼠脑组织JAK2、STAT3、VEGF mRNA表达水平.结果 模型复制后4 h,电针组、模型组大鼠神经功能评分显著高于假手术组和正常组(P<0.05);与假手术组比较,模型组JAK2、p-JAK2、STAT3、p-STAT3、VEGF蛋白表达水平均显著升高(P<0.05),蛋白表达水平显著升高(P<0.05);JAK2、STAT3、VEGF mRNA表达水平显著升高(P<0.05);与模型组比较,电针组7 d后神经功能评分显著降低(P<0.05);STAT3、VEGF、p-STAT3、JAK2、p-JAK2蛋白表达水平显著升高(P<0.05);JAK2、STAT3、VEGF mRNA表达水平显著升高(P<0.05).结论 电针可能通过激活JAK2/STAT3通路、促进下游VEGF的表达发挥对局灶性脑缺血大鼠的神经保护作用.
Abstract
Objective To investigate the effect of electroacupuncture at Baihui and Dazhui points on the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3)pathway and vascular endothelial growth factor (VEGF),as well as its neuroprotective mechanism in rats with focal cerebral ischemia.Methods A total of 36 Sprague-Dawley rats were randomly divided into normal group,sham-operation group,model group,and electroacupuncture group,with 9 rats in each group.The Longa suture method was used to establish a model of focal cerebral ischemia,and the rats in the electroacupuncture group were given electroacupuncture at 4 hours after modeling,for 7 days in total.After modeling,the groups were compared in terms of neurological deficit score at 4 hours and 7 days after surgery;Western blot was used to measure the protein expression levels of JAK2,phosphorylated JAK2 (p-JAK2),STAT3,phosphorylated STAT3 (p-STAT3),and VEGF in brain tissue,and RT-PCR was used to measure the mRNA expression levels ofJAK2,STAT3,and VEGF in brain tissue.Results At 4 hours after modeling,the electroacupuncture group and the model group had a significantly higher neurological deficit score than the sham-group and the normal group (P<0.05).Compared with the sham-operation group,the model group had significant increases in the protein expression levels of JAK2,p-JAK2,STAT3,p-STAT3 and VEGF (P<0.05)and the mRNA expression levels of JAK2,STAT3,and VEGF (P<0.05).Compared with the model group,the electroacupuncture group had a significant re-duction in neurological deficit score after 7 days (P<0.05)and significant increases in the protein expression levels of STAT3, VEGF,p-STAT3,JAK2,and p-JAK2 (P<0.05)and the mRNA expression levels of JAK2,STAT3,and VEGF (P<0.05).Conclusion Electroacupuncture exerts a neuroprotective effect on rats with focal cerebral ischemia possibly by activa-ting the JAK2/STAT3 pathway and promoting the expression of downstream VEGF.
基金项目
安徽中医药大学省级新时代育人质量工程(研究生教育)项目(2022tsxwd040)
安徽高等学校自然科学研究重点项目(KJ2021A0573)
安徽高等学校自然科学研究重点项目(KJ2021A0576)
维普
万方数据