Mechanism of Action of New Changyu Enema Prescription in Treatment of Ulcerative Colitis:A Study Based on Network Pharmacology and Experimental Validation
Objective To investigate the mechanism of action of New Changyu Enema Prescription in the treatment of ulcera-tive colitis (UC).Methods Related data platforms were used to obtain the effective constituents of the Chinese herbal medicine in New Changyu Enema Prescription,and GeneCards was used to obtain the targets associated with UC and construct a tradi-tional Chinese medicine-effective constituent-target network.String database was used to construct a protein-protein interaction network,and Metascape database was used to perform gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses.Weishengxin platform was used to plot bar charts and bubble charts for visualization,and molecular doc-king was used to validate the affinity between the effective constituents and targets.Animal experiments were conducted to ob-serve the therapeutic effect of New Changyu Enema Prescription on UC and the regulation of related targets.Results A total of 96 effective constituents were obtained,with quercetin,kaempferol,luteolin,beta-sitosterol,isocorypalmine,and form-ononetin as the key effective constituents,and a total of 1214 targets were obtained for UC,with IL-6,TP53,VEGFA,JUN, IL-1β,PTGS2,and ESR1 as core targets.The KEGG analysis identified the important roles of fluid shear stress and the signa-ling pathways of atherosclerosis and AGE-RAGE in diabetic complications.Molecular docking showed good affinity between the key effective constituents and the core targets,and animal experiments showed that New Changyu Enema Prescription could regulate related targets and effectively improve intestinal inflammation.Conclusion New Changyu Enema Prescription exerts a therapeutic effect on UC through targeted regulation of fluid shear stress and the signaling pathways including atherosclerosis.
New Changyu Enema PrescriptionUlcerative colitisNetwork pharmacologyExperimental validation
万方数据
维普
