Chemical Components of Different Polar Extracts of Dendrobium officinale Water Extract:An Analysis Based on Ultra-performance Liquid Chromatography-Linear Ion Trap Quadrupole-Orbitrap-Mass Spectrometry
Objective To establish a method based on ultra-performance liquid chromatography-linear ion trap quadrupole-Orbi-trap-mass spectrometry (UPLC-Q-Orbitrap-MS)for qualitative characterization of the extraction components of petroleum e-ther,ethyl acetate,and n-butanol in water extracts of the traditional Chinese medicine Dendrobium officinale.Methods The water extracts of Dendrobium officinale were obtained and sequentially extracted with petroleum ether,ethyl acetate,and n-butanol,and the extraction components of petroleum ether,ethyl acetate,and n-butanol were obtained after concentration by rotary evaporation.The samples were analyzed and processed by UPLC-Q-Orbitrap-MS to determine the information of com-pounds based on elemental composition,molecular weight,mass-to-charge ratio,and fragment ions and to deduce the cleavage pattern,and the compounds were identified by comparison with the literature,MzCloud,MzVault,PudChem,and local data-bases.Results A total of 62 compounds were identified in positive and negative ionmodes,including 20 flavonoid components, 6 bibenzyl components,6 carboxylic acids and their derivatives,6 fatty acids and their derivatives,4 phenolic components,3 a-mino acids and their derivatives,2 glycan components,1 each of alkaloids,phenylpropanoids,anthraquinones,and terpenoids, and 3 other types of components,and 8 unknown components were also detected with a relatively higher level and strong mass spectrometry signals.Seven compounds were identified in the petroleum ether extract,36 compounds were identified in the eth-yl acetate extract,and 48 compounds were identified in the n-butanol extract.By comparison with the literature,among these 62 compounds,7 were reported for the first time from Dendrobium officinale.Conclusion This method can rapidly and ef-fectively analyze multiple chemical components in Dendrobium officinale,which can provide a reference for the quality control of Dendrobium officinale and the basic research on medicinal substances.
Dendrobium officinaleUltra-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometryChemical componentsQualitative analysis
万方数据
维普
