Role and Molecular Mechanism of Naoluoxintong Decoction in Regulating Inflammation in Ischemic Stroke Based on Weighted Gene Co-expression Network Analysis
Objective To investigate the role and molecular mechanism of Naoluoxintong Decoction in regulating inflammation in ischemic stroke (IS)using weighted gene co-expression network analysis (WGCNA).Methods GEO database was used to ob-tain the transcriptome data of IS patients,limma was used for the analysis of differentially expressed genes,and WGCNA was used to obtain IS-related targets.The active components of Naoluoxintong Decoction were obtained,and their action targets were predicted.The targets identified by WGCNA were intersected with the predicted action targets,and molecular docking was performed for the key components and targets.Results The WGCNA analysis identified five key targets for the active components of Naoluoxintong Decoction,i.e.,GLRX,ELANE,S100A8,BCL2A1,and CAMP.The KEGG enrichment a-nalysis showed that Naoluoxintong Decoction mainly exerted an anti-IS effect by regulating the IL-17 and NF-κB signaling path-ways.Molecular docking validated the core targets with good binding activities to the key compounds.Conclusion Naoluoxin-tong Decoction exerts a therapeutic effect on IS through multiple components,targets,and pathways,possibly by regulating the IL-17 and NF-κB inflammatory signaling pathways through the main active components of Naoluoxintong Decoction.