Rapid Analytical Identification of Ethanol-soluble Components of Ganoderma sinense Based on Ultra Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry
Objective To perform rapid identification and analytical identification of the ethanol-soluble components of Gano-derma sinense by using ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Orbitrap Exploris 120).Methods UPLC was performed on a Waters ACQUITY UPLC BEH C18column (1.7μm,150mm × 2.1 mm)with A (0.1% formic acid water)-B (acetonitrile)as the mobile phase for gradient elution at a column temperature of 35 ℃,a flow rate of 0.2 mL/min,and an injection volume of 3μL.The mass spectrometry data of eth-anol-soluble components were collected in the negative ion mode,and the ethanol-soluble components of Ganoderma sinense were analyzed based on the precise relative molecular mass of primary mass spectrometry,the characteristic fragments of sec-ondary mass spectrometry,and related articles.Results In this experiment,a total of 16 components were preliminarily iden-tified in the alcohol extract of the fruiting body of Ganoderma sinense by Compound Discover 3.3 software,literature review,and manual proofreading,among which there were 12 triterpenes and 4 fatty acids.Conclusion A relatively complete and con-venient method is established to explore the differences in the material basis of Ganoderma sinense,which provides a certain theoretical basis for further research on the differences in the pharmacodynamic material basis and its mechanism of action and theoretical support for identifying the components of Ganoderma lucidum and establishing quality control standards.
Ganoderma sinenseUltra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometryEthanol-soluble componentsTriterpenes