安徽中医药大学学报2024，Vol.43Issue(6) ：94-100.DOI:10.3969/j.issn.2095-7246.2024.06.019

UPLC-Orbitrap-MS法快速分析鉴定紫芝醇溶性成分

Rapid Analytical Identification of Ethanol-soluble Components of Ganoderma sinense Based on Ultra Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry

崩淑娟 李思雨 张静 罗文慧 韩海伦 熊清平 彭灿
安徽中医药大学学报2024，Vol.43Issue(6) ：94-100.DOI:10.3969/j.issn.2095-7246.2024.06.019
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  • 维普
  • 万方数据

UPLC-Orbitrap-MS法快速分析鉴定紫芝醇溶性成分

Rapid Analytical Identification of Ethanol-soluble Components of Ganoderma sinense Based on Ultra Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry

崩淑娟 1李思雨 2张静 1罗文慧 1韩海伦 2熊清平 3彭灿4
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作者信息

  • 1. 安徽中医药大学新安医学与中医药现代化研究所,安徽 合肥 230012;安徽中医药大学药学院,安徽 合肥 230012;安徽中医药大学安徽省现代中药产业共性技术研究中心,安徽 合肥 230012
  • 2. 安徽中医药大学药学院,安徽 合肥 230012
  • 3. 安徽中医药大学新安医学与中医药现代化研究所,安徽 合肥 230012;淮阴工学院,江苏 淮安 223001
  • 4. 安徽中医药大学新安医学与中医药现代化研究所,安徽 合肥 230012;安徽中医药大学药学院,安徽 合肥 230012;安徽中医药大学安徽省现代中药产业共性技术研究中心,安徽 合肥 230012;安徽中医药大学安徽省乡村振兴协同技术服务中心,安徽 合肥 230012;省部共建安徽道地中药材品质提升协同创新中心,安徽 合肥 230012
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摘要

目的 采用超高效液相色谱—四极杆—静电场轨道阱高分辨质谱系统对紫芝的醇溶性成分进行快速识别并分析鉴定.方法 采用Waters ACQUITY UPLC BEH C18色谱柱(1.7μm,150 mm×2.1 mm),以A(0.1％甲酸水)—B(乙腈)为流动相进行梯度洗脱,柱温为35℃,流速为0.2 mL/min,进样容积为3μL.在负离子模式下采集紫芝醇溶性的质谱数据,根据一级质谱的精确相对分子质量、二级质谱特征碎片,结合文献分析紫芝的醇溶性成分.结果 通过Compound Discover 3.3软件、文献参考和人工校对的方法对紫芝醇提物共初步鉴定了16种成分.其中12种三萜类成分,4个脂肪酸类成分.结论 所建立的方法完整、便捷,可为紫芝的药效物质基础差异及其作用机制研究提供一定的理论依据,并为紫芝成分鉴定及其质量控制标准提供理论支撑.

Abstract

Objective To perform rapid identification and analytical identification of the ethanol-soluble components of Gano-derma sinense by using ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Orbitrap Exploris 120).Methods UPLC was performed on a Waters ACQUITY UPLC BEH C18column (1.7μm,150mm × 2.1 mm)with A (0.1％ formic acid water)-B (acetonitrile)as the mobile phase for gradient elution at a column temperature of 35 ℃,a flow rate of 0.2 mL/min,and an injection volume of 3μL.The mass spectrometry data of eth-anol-soluble components were collected in the negative ion mode,and the ethanol-soluble components of Ganoderma sinense were analyzed based on the precise relative molecular mass of primary mass spectrometry,the characteristic fragments of sec-ondary mass spectrometry,and related articles.Results In this experiment,a total of 16 components were preliminarily iden-tified in the alcohol extract of the fruiting body of Ganoderma sinense by Compound Discover 3.3 software,literature review,and manual proofreading,among which there were 12 triterpenes and 4 fatty acids.Conclusion A relatively complete and con-venient method is established to explore the differences in the material basis of Ganoderma sinense,which provides a certain theoretical basis for further research on the differences in the pharmacodynamic material basis and its mechanism of action and theoretical support for identifying the components of Ganoderma lucidum and establishing quality control standards.

关键词

紫芝/超高效液相色谱—四极杆—静电场轨道阱高分辨质谱/醇溶性成分/三萜类

Key words

Ganoderma sinense/Ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry/Ethanol-soluble components/Triterpenes

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出版年

2024
安徽中医药大学学报
安徽中医学院

安徽中医药大学学报

CSTPCD
影响因子：0.796
ISSN：2095-7246
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