Study on Callus Induction Culture of Lepidium meyenii Seeds and Roots
Lepidiwm meyenii seed and root callus induction culture was tested by proliferation medium and the medium leading to early screening and anti - browning treatment. The results showed the optimal conditions: to seed or root the optimal combination of the early guide media were 3/4MS + 0. 3mg · L-1 6 - BA + 0. 3mg · L-1 NAA + 25 g · L-1 sucrose, MS + 0.3 mg · L-1 6 - BA + 0.3mg · L-1 NAA + 25 g · L-1 sucrose. The proliferation of seed and spikelet optimal combination of media were MS + (0. 3 ~0. 5 mg · L-1 ) 6 - BA +0. 3 mg · L-1 NAA, MS + 0. 3mg · L-1 6 -BA +0.3 mg · L-1 NAA. The browning control could be done under certain culture conditions. The study of provided technical basis for the breeding.
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维普
