北京草莓种植区土壤微生物群落结构解析
Analysis of soil microbial community structure in strawberry planting areas in Beijing
曾文 1李强 2马毅楠 1马荣 1张晓霞 1李世贵 1魏海雷 1谷医林1
作者信息
- 1. 中国农业科学院农业资源与农业区划研究所,北方干旱半干旱耕地高效利用全国重点实验室,农业农村部农业微生物资源收集与保藏重点实验室,北京 100081
- 2. 山东土大厨肥业有限公司,山东济宁 272300
- 折叠
摘要
为了解北京草莓种植区土壤微生物组群落动态与土壤质量的关系,本研究利用高通量测序,系统分析北京地区草莓根际微生物组.结果显示,微生物群落结构分布具有明显的区域性,磷元素和钾元素是影响草莓根际菌群组成最主要的因子;扩增子测序分析表明,健康植株比发病植株根际土壤微生物群落结构更稳定;网络分析发现了潜在的与草莓土壤健康相关的菌属,发病样品中链霉菌属(Streptomyces)和芽胞杆菌属(Bacillus)占据重要位置.进一步对比健康草莓与发病根际微生物组成的差异,探究了病害发生与根际微生物群落变化之间的规律,并通过构建相关性网络发现了潜在的病害防治菌属,为北京草莓土传病害预警和防控奠定了基础.
Abstract
To understand the relationship between soil microbial community dynamics and soil quality in strawberry planting areas in Beijing,this study used high-throughput sequencing to systematically analyze the rhizosphere microbi-ome of strawberries in the Beijing area.The results showed that the microbial composition has obvious regional character-istics,and phosphorus and potassium elements are the most important factors affecting the composition of strawberry rhi-zosphere microbiota.Amplicon sequencing analysis showed that healthy plants have more stable microbial community structure in the rhizosphere soil than diseased plants.Network analysis identified potential bacterial genera associated with strawberry soil health,with Streptomyces and Bacillus occupying significant positions in the diseased samples.We further compared the differences in rhizosphere microbial composition between healthy and diseased strawberries,explored the patterns between disease occurrence and changes in rhizosphere microbial communities,and identified potential disease control genera by constructing co-occurrence networks.This study lays the foundation for early warning and prevention of soilborne strawberry diseases in Beijing.
关键词
扩增子测序/微生物组/草莓枯萎病/根际微生物/土壤理化性质Key words
amplicon sequencing/microbiome/strawberry fusarium wilt/rhizosphere microorganism/soil physio-chemical property引用本文复制引用
基金项目
特色作物产业技术体系北京市创新团队(BAIC04-2023)
北京市乡村振兴科技项目(BJXCZX2022)
出版年
2024
国家科技期刊平台
NSTL
万方数据