Multifunctional characterization and genomic analysis of Pseudomonas taetrolens P683
Pseudomonas has outstanding potential in biological control of plant diseases because of its broad-spec-trum antibacterial activity.In this study,a strain of Pseudomonas taetrolens P683 was tested for its disease prevention ability,pathogen broad-spectrum resistance and plant growth promoting index.The genes related to potential secondary metabolites were further analyzed by genome sequencing,functional annotation and antiSMASH prediction.The results showed that strain P683 could control tobacco wildfire disease effectively.Strain P683 could antagonize broad-spectrum pathogenic bacteria including Ralstonia solanacearum,Clavibacter michiganensis,Xanthomonas oryzae pv.oryzae,Xanthomonas oryzae pv.oryzicola,Xanthomonas campestris pv.carnpestris,Pseudomonas syringae pv.tomato,Sal-monella sp.,and Staphylococcus aureu.Plate coculturing showed that strain P683 had stronger competitive capacity than Bacillus subtilis.Plant growth promoting index detection showed that strain P683 could hydrolyze inorganic and organic phosphorus,produce siderophore,protease,cellulase,IAA,and NH3.Genome sequencing revealed that the genome size of strain P683 is 4.96 Mbp,encoding 4 477 proteins.Seven secondary metabolite biosynthetic gene clusters were predicted by antiSMASH,encoding products containing lactone protease inhibitors and arylpolyenes that may exert anti-bacterial activity.This study lays the foundation for further analysis of the bactericidal mechanism of strain P683 and de-velopment of strain P683 as a new biocontrol agent.
NETL
NSTL
万方数据
