Isolation and identification of foliar endophytic fungi of Picea crassifolia in the Qilian Mountains
Endophytic fungi play important roles in plant growth,development,and stress resistance.Picea crassifo-lia,an endemic plant with great ecological value in China,serves as the primary tree species in the Qilian Mountains of China.However,there has been limited research on its foliar endophytic fungi.In this study,the culturable foliar endo-phytic fungi of P.crassifolia were isolated using tissue separation methods.Subsequently,these endophytic fungi were identified based on the morphological characteristics of their colonies and the rDNA ITS sequence analysis.The results showed that 32 fungal strains were isolated from the needles of P.crassifolia,belonging to 12 species,12 genera,11 families,6 orders,4 classes of 1 phylum(Ascomycota).Among them,Torula camporesii had the highest isolation fre-quency(18.75％),followed by Leptosphaerulina australis(15.63％).Among 32 fungal strains,14 were identified as pathogens of various plants,including 2 strains of Cytospora piceae,which are pathogens of P.crassifolia twig blight.This study revealed the composition of culturable foliar endophytic fungi of P.crassifolia and obtained fungal resources with potential research value.The research findings serve as a significant supplement to related studies on P.crassifolia in the Qilian Mountains,providing a theoretical foundation for further research on the potential ecological and application value of endophytic fungi of P.crassifolia.
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