Preliminary investigation and analysis of rice virus diseases and their pathogens in Tibet
There are a variety of crop planting areas in Chayu County and Medog County in the low-altitude areas of Tibet Autonomous Region,among which rice is one of the main breeding crops.The existence of rice virus seriously threatens rice yield,so it is necessary to systematically,scientifically and accurately grasp the types and occurrence of rice virus diseases in Tibet.In this study,rice samples suspected of virus infection were collected from Chayu County and Me-dog County of Nyingchi City,Tibet Autonomous Region for the first time,and rice virus species in Tibet were analyzed by RT-PCR.The experimental results showed that there were mainly six kinds of rice viruses in Tibet,namely rice dwarf virus(RDV),rice stripe virus(RSV),rice hoja blanca virus(RHBV),rice necrosis mosaic virus(RNMV),rice ragged stunt virus(RRSV),and southern rice black streaked dwarf virus(SRBSDV).Among them,RDV had the high-est detection rate,the widest spread,and the most serious harm,even causing crop failure in some fields.The results of this survey give us a preliminary understanding of the infection of rice virus diseases in Tibet,and lay a solid foundation for further exploring the occurrence and control of rice virus diseases in the region and improving rice yield in the future.