Effects of a dominant fungus LW-1 on soil available nitrogen,available phosphorus,soil enzyme activity and biological availability of cadmium in rice-fishery co-cropping field
To investigate the effects of dominant fungi from rice-loach-frog co-culture farmland on soil available nitro-gen,available phosphorus,soil enzyme activities and cadmium bioavailability in cadmium-contaminated soil.this study isolated,purified and preliminarily identified the dominant fungus LW-1 from the soil of rice-loach-frog co-culture farm-land,and then prepared three concentrations of spore suspensions of 1×103,1×105,and 1×107 spores/mL,which were added to soils contaminated with 0,5,10 and 20 mg/kg cadmium,respectively.Soil samples were collected every 10 days to measure soil available phosphorus,available nitrogen,invertase,catalase,urease and available cadmium con-tents.The results showed that with the increase of spore suspension concentration,the contents of available nitrogen and available phosphorus in soil increased significantly(P<0.05),the activities of soil invertase and urease increased signifi-cantly(P<0.05),and the content of available cadmium decreased significantly(P<0.05).This indicates that the domi-nant fungus from rice-loach-frog co-culture farmland can improve soil fertility and reduce the bioavailability of cadmium in cadmium-contaminated soil.The research results provide a theoretical basis for the application of this mode in soil im-provement and remediation,and also provide a new perspective for understanding the mechanism of soil fertility in rice-loach-frog co-cropping farmland higher than that in conventional paddy field.