Isolation of actinomycetes from rhizosphere soil of Populus euphratica and screening of their antagonistic activity
Rhizosphere soil microorganisms are crucial in resisting the invasion of pathogenic microorganisms and en-hancing plant disease resistance.Populus euphratica,a commonly found plant in desert areas,possesses a diverse range of rhizosphere soil microorganisms and holds significant research value.This study aims to investigate the microbial inter-actions in the rhizosphere soil of Populus euphratica and enrich the extreme environmental antagonistic microbial strain li-brary.The diversity of actinomycete resources in 10 different regions of Populus euphratica rhizosphere soil in southern Xinjiang was analyzed using the selective medium screening method.The strains were subjected to 16S rDNA sequencing and plate confrontation method for screening.The results revealed the isolation and screening of a total of 269 strains,with 78 strains exhibiting good growth status selected for analysis and identification.These strains were classified into 5 genera,with the genus Streptomyces being the dominant group.Furthermore,32 strains of active actinomycetes were screened for antagonistic activity,and targeting pathogens included Candida albicans,Alternaria tenuissima,Verticilli-um dahliae,Fusarium oxysporum,Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneu-moniae,Erwinia amylohydrolylic,and Salmonella typhi.These findings contribute to the enrichment of microbial re-sources and provide a microbial strain foundation for the prevention and control of major agricultural and animal husbandry diseases in Xinjiang.
Populus euphratica rhizosphere soilactinomycetespecies diversityantimicrobial activity