Regurgitation behavior and chemical composition detection of regurgitant of the larvae of Acanthacorydalis asiatica
The regurgitation behavior of the larvae of Acanthacorydalis asiatica was observed and described,and the chemical components of regurgitant and intestinal fluid were detected.According to the studies,when A.asiatica lar-vae are stimulated,they are curling up,attacking and swinging.In critical moments,they would spit out a yellow-brown foul-smelling liquid from their mouths to attack enemies and escape.It is found by observing the digestive tract of the first and last instar larvae and simulating the intestinal movement that the regurgitation processes of A.asiatica are as follows:the cardiac valve is opened with the pressure generated from the frontal stomach relaxation,enabling the fluid in the mid-gut to return to the esophagus and crop of the foregut.Then,the fluid is returned to the pharynx through the contraction of the front stomach and esophagus,the dorsal pharyngeal expander and the muscles on both sides control the pharynx,and the intestinal fluid is spat out from the mouth to stimulate the enemy,finally retreating quickly to escape.The chemi-cal components of the metabolites in the oral regurgitant and foregut and midgut fluid of the A.asiatica larvae were detect-ed using LC-MS method.The top 25 metabolites were screened from the positive and negative ions with big differences in the oral regurgitant and intestinal fluid based on the peak area integral value ratio of the two samples,and it was found that there were 12 stimulating substances,16 toxic substances and 3 special taste substances among these metabolites.These substances can effectively defend against natural enemies.
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