Genetic diversity analysis of Glyptosternum maculatum in the middle and upper reaches of the Yarlung Zangbo River based on mitochondrial Cyt b gene and D-loop region sequence
The analysis of the genetic diversity of Glyptosternum maculatum in the middle and upper reaches of the Yarlung Zangbo River in Xizang can provide theoretical basis for its conservation and artificial breeding,the cytochrome b(Cyt b)gene and D-loop region sequences were amplified and sequenced to analyze the correlation between its mitochon-drial sequence characteristics and the genetic diversity of the population.The findings revealed that:the Cyt b gene se-quence length was 1 138 bp,with a higher A+T content(59.5％)compared to C+G(40.5％);32 mutation sites were identified,including 26 single mutation sites and 6 simplified information sites;a total of 21 haplotypes were identified,with Hap 1 having the highest number of individuals(69.44％);population haplotype diversity(Hd)was measured to be 0.509±0.050,nucleotide diversity(Pi)was 0.000 71±0.000 11,and Tajima's D neutral test value was-2.532 91(P＜0.001).The findings also revealed that:the D-loop sequence was 893 bp long,with a higher A+T content(64.31％)than C+G(35.69％);35 mutation sites were detected,including 21 single mutation sites and 14 simplified information sites;23 haplotypes were identified,Hd was 0.701±0.028,Pi was 0.001 67±0.000 28,haplotypes Hap 1 and Hap 3 were more prevalent(45.14％and 30.56％respectively);Tajima's D neutral test value for the D-loop gene was-2.366 38,indicating extremely significant negative values(P＜0.01)and suggesting population expansion in this popu-lation.The Cyt b gene and D-loop region sequence of the population are suitable for intraspecific genetic analysis,and the D-loop region has more evolutionary advantages.The genetic diversity of the population is relatively low,which has cer-tain reference value for the genetic breeding and protection of G.maculatum.
Yarlung Zangbo RiverGlyptosternum maculatumgenetic diversitycytochrome b(Cyt b)geneD-loop region
万方数据
维普
